Myc tag antibody [GT0002] Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-Myc tag antibody [GT0002] (GTX628259). (A) Input, 20ug of HeLa transfected lysates. The precipitated protein was detected by GTX628259 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit (GTX221667-01) was used in Western blot.

Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with Myc tag antibody [GT0002] (GTX628259) (1 μg/mL). Goat antibody Mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Myc tag antibody [GT0002] (GTX628259) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Myc tag antibody [GT0002] detects Myc tag protein at cell membrane by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Myc tag stained by Myc tag antibody [GT0002] (GTX628259) diluted at 1:2000.