*Optimal dilutions/concentrations should be determined by the researcher.
Application
Recommended Dilution
WB
1:5000-1:20000
ICC/IF
1:100-1:2000
IP
1:100-1:500
Not tested in other applications.
Positive Control
BNP8B-Myc-DDk-tag tranfected 293T
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
The immunogen used to generate this antibody corresponds to Myc tag
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888026
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Myc tag antibody [GT0002] Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-Myc tag antibody [GT0002] (GTX628259). (A) Input, 20ug of HeLa transfected lysates. The precipitated protein was detected by GTX628259 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit (GTX221667-01) was used in Western blot.
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GTX628259 ICC/IF Image
Myc tag antibody [GT0002] detects Myc tag protein at cell membrane by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Myc tag stained by Myc tag antibody [GT0002] (GTX628259) diluted at 1:2000.
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GTX628259 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Myc tag antibody [GT0002] (GTX628259) diluted at 1:5000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.