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Myc tag antibody [GT0002]

Anti-Myc tag antibody [GT0002] used in Immunoprecipitation (IP). GTX628259
Anti-Myc tag antibody [GT0002] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628259
Anti-Myc tag antibody [GT0002] used in Western Blot (WB). GTX628259

Cat. No. GTX628259

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT0002

Isotype

 IgG1

Application

 WB, ICC/IF, IP
Package
100 μl ($399),
25 μl ($169)
See all Myc tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:2000
IP 1:100-1:500
Not tested in other applications.

Positive Control

BNP8B-Myc-DDk-tag tranfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Myc tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888026

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Myc tag antibody

Research Area

DATA IMAGES

Anti-Myc tag antibody [GT0002] used in Immunoprecipitation (IP). GTX628259

GTX628259 IP Image

Myc tag antibody [GT0002] Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-Myc tag antibody [GT0002] (GTX628259). (A) Input, 20ug of HeLa transfected lysates. The precipitated protein was detected by GTX628259 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit (GTX221667-01) was used in Western blot.

Anti-Myc tag antibody [GT0002] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628259

GTX628259 ICC/IF Image

Myc tag antibody [GT0002] detects Myc tag protein at cell membrane by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Myc tag stained by Myc tag antibody [GT0002] (GTX628259) diluted at 1:2000.

Anti-Myc tag antibody [GT0002] used in Western Blot (WB). GTX628259

GTX628259 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Myc tag antibody [GT0002] (GTX628259) diluted at 1:5000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.

REFERENCE

Application Reference

Gomez R et al. EMBO Rep 2016; 17 (5) : 695-707 Sororin loads to the synaptonemal complex central region independently of meiotic cohesin complexes.
Application : ICC/IF
Lin W et al. EMBO J 2013; 32 (10) : 1425-39 Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is required for telomere integrity.
Application : WB
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations