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Myc tag antibody

Anti-Myc tag antibody used in Immunoprecipitation (IP). GTX115046
Anti-Myc tag antibody used in Western Blot (WB). GTX115046
Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046
Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046
Anti-Myc tag antibody used in Western Blot (WB). GTX115046
Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046

Cat No. GTX115046

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IP 1:10000
Not tested in other applications.

Positive Control

293T transfected myc-tag recombinant protein

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic peptide corresponding to a region within amino acids 410 and 419 of human c-Myc

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169757

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Myc tag antibody

Research Area

DATA IMAGES

Anti-Myc tag antibody used in Immunoprecipitation (IP). GTX115046

GTX115046 IP Image

Myc antibody Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-Myc antibody (GTX115046). (A) Input, 15ug of HeLa transfected lysates. The precipitated protein was detected by GTX115046 diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit (GTX225856-01) was used in Western blot.

Anti-Myc tag antibody used in Western Blot (WB). GTX115046

GTX115046 WB Image

Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing Myc tagged protein, using Myc tag antibody (GTX115046) diluted at 1:10000.
A: Non-transfected 293T lysate (10 μg)
B: Transfected 293T lysate (10 μg) (~60 kDa)
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046

GTX115046 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Myc tag transfected HeLa, using Myc tag(GTX115046) antibody (green) at 1:3000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2500.

Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046

GTX115046 ICC/IF Image

Myc tag antibody detects Myc tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Myc tag stained by Myc tag antibody (GTX115046) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Myc tag antibody used in Western Blot (WB). GTX115046

GTX115046 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Myc tag antibody (GTX115046) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046

GTX115046 ICC/IF Image

Myc tag antibody detects Myc tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Myc-tag tagged protein stained by Myc tag antibody (GTX115046) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

Myc tag antibody Cat No. GTX115046

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Oncogene in 2017. PMID: 28436950
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2016. PMID: 27489281
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169