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Myc tag antibody

Anti-Myc tag antibody used in Western Blot (WB). GTX20172

Cat. No. GTX20172

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IP, ELISA, IHC
Package
50 μg ($399)
See all Myc tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC 1:100-1:250
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Chickens were immunized with a synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) conjugated to keyhole limpet hemocyanin.

Purification

Purified by antigen-affinity chromatography
Antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a SulfoLinkÆ (Pierce) column conjugated with the peptide used for immunizations, and then dialyzed against PBS. Filter-

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.

Research Area

DATA IMAGES

Anti-Myc tag antibody used in Western Blot (WB). GTX20172

GTX20172 WB Image

WB analysis of SAP1a cDNA fused with a c-myc epitope tag transfected cell using GTX20172 Myc tag antibody.
Lane 1 : 30 ug protein
Lane 2 : 20 ug protein
Lane 3 : 10 ug protein
Lane 4 : 5 ug protein

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399