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Myelin basic Protein antibody [GT3412]

Anti-Myelin basic Protein antibody [GT3412] used in Western Blot (WB). GTX634292
Anti-Myelin basic Protein antibody [GT3412] used in IHC (Frozen sections) (IHC-Fr). GTX634292
Anti-Myelin basic Protein antibody [GT3412] used in IHC (Paraffin sections) (IHC-P). GTX634292

Cat No. GTX634292

Host Mouse
Clonality Monoclonal
Clone Name GT3412
Isotype IgG1
Application WB, IHC-P, IHC-Fr
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

mouse brain
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Myelin basic Protein. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

myelin basic protein,C76307,Hmbpr,R75289,golli-mbp,jve,mld,shi

Cellular Localization

Isoform 13: Myelin membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 12: Myelin membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 11: Myelin membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 10: Myelin membr

Background

The protein encoded by the classic Mbp gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, Mbp-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long Mbp gene (otherwise called "Golli-Mbp") that contains 3 additional exons located upstream of the classic Mbp exons. Alternative splicing from the Golli and the Mbp transcription start sites gives rise to 2 sets of Mbp-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-Mbp, spliced in-frame to 1 or more Mbp exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to Mbp aa sequence. The second family of transcripts contain only Mbp exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the Mbp transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Mutation of the Mbp gene is associated with the 'shiverer' and 'myelin deficient' phenotypes in mouse. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Myelin basic Protein antibody [GT3412] used in Western Blot (WB). GTX634292

GTX634292 WB Image

Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Myelin basic Protein antibody [GT3412] (GTX634292) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Myelin basic Protein antibody [GT3412] used in IHC (Frozen sections) (IHC-Fr). GTX634292

GTX634292 IHC-Fr Image

Myelin basic Protein antibody [GT3412] detects Myelin basic Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Myelin basic Protein stained by Myelin basic Protein antibody [GT3412] (GTX634292) diluted at 1:500.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Myelin basic Protein antibody [GT3412] used in IHC (Paraffin sections) (IHC-P). GTX634292

GTX634292 IHC-P Image

Myelin basic Protein antibody [GT3412] detects Myelin basic Protein protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Myelin basic Protein antibody [GT3412] (GTX634292) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169