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Myelin basic Protein antibody

Anti-Myelin basic Protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108
Anti-Myelin basic Protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108
Anti-Myelin basic Protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108
Anti-Myelin basic Protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108
Anti-Myelin basic Protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108
Anti-Myelin basic Protein antibody used in Western Blot (WB). GTX133108

Cat No. GTX133108

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr, IHC
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Myelin Basic Protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

myelin basic protein,C76307,Hmbpr,R75289,golli-mbp,jve,mld,shi

Cellular Localization

Isoform 13: Myelin membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 12: Myelin membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 11: Myelin membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 10: Myelin membr

Background

The protein encoded by the classic Mbp gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, Mbp-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long Mbp gene (otherwise called "Golli-Mbp") that contains 3 additional exons located upstream of the classic Mbp exons. Alternative splicing from the Golli and the Mbp transcription start sites gives rise to 2 sets of Mbp-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-Mbp, spliced in-frame to 1 or more Mbp exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to Mbp aa sequence. The second family of transcripts contain only Mbp exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the Mbp transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Mutation of the Mbp gene is associated with the 'shiverer' and 'myelin deficient' phenotypes in mouse. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Myelin basic Protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108

GTX133108 IHC-Fr Image

Myelin Basic Protein antibody detects Myelin Basic Protein protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Myelin Basic Protein protein stained by Myelin Basic Protein antibody (GTX133108) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Myelin basic Protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

GTX133108 IHC-P Image

Myelin Basic Protein antibody detects Myelin Basic Protein protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Myelin Basic Protein antibody (GTX133108) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Myelin basic Protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

GTX133108 IHC-P Image

Myelin Basic Protein antibody detects Myelin Basic Protein protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Myelin Basic Protein antibody (GTX133108) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Myelin basic Protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108

GTX133108 IHC-Fr Image

Myelin Basic Protein antibody detects Myelin Basic protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: Myelin Basic protein stained by Myelin Basic Protein antibody (GTX133108) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

Anti-Myelin basic Protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108

GTX133108 IHC-Fr Image

Myelin Basic Protein antibody detects Myelin Basic Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Myelin Basic Protein stained by Myelin Basic Protein antibody (GTX133108) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Myelin basic Protein antibody used in Western Blot (WB). GTX133108

GTX133108 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Myelin Basic Protein antibody (GTX133108) diluted at 1:10000.

REFERENCE
REVIEW

Myelin basic Protein antibody Cat No. GTX133108

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 23-Nov-2016
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : mouse brain tissue
Blocking : 0.25% TX-100, 4% normal goat serum, 20°C, 2Hr
Fixation : Free floating sections, immunofluorescence
Permeabilization /Antigen retrieval : 0.25% TX-100
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169