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Myelin basic protein antibody [HL1033]

Recombinant antibody
Anti-Myelin basic protein antibody [HL1033] used in Western Blot (WB). GTX635873
Anti-Myelin basic protein antibody [HL1033] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635873
Anti-Myelin basic protein antibody [HL1033] used in IHC (Paraffin sections) (IHC-P). GTX635873
Anti-Myelin basic protein antibody [HL1033] used in IHC (Paraffin sections) (IHC-P). GTX635873
Anti-Myelin basic protein antibody [HL1033] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635873
Anti-Myelin basic protein antibody [HL1033] used in Western Blot (WB). GTX635873
Anti-Myelin basic protein antibody [HL1033] used in IHC (Frozen sections) (IHC-Fr). GTX635873
Anti-Myelin basic protein antibody [HL1033] used in IHC (Frozen sections) (IHC-Fr). GTX635873

Cat No. GTX635873

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1033

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:500000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Myelin basic Protein. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909911

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myelin basic protein , C76307 , Hmbpr , R75289 , golli-mbp , jve , mld , shi

Cellular Localization

Nucleus

Background

The protein encoded by the classic Mbp gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, Mbp-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long Mbp gene (otherwise called "Golli-Mbp") that contains 3 additional exons located upstream of the classic Mbp exons. Alternative splicing from the Golli and the Mbp transcription start sites gives rise to 2 sets of Mbp-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-Mbp, spliced in-frame to 1 or more Mbp exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to Mbp aa sequence. The second family of transcripts contain only Mbp exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the Mbp transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Mutation of the Mbp gene is associated with the 'shiverer' and 'myelin deficient' phenotypes in mouse. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Myelin basic protein antibody [HL1033] used in Western Blot (WB). GTX635873

GTX635873 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Myelin basic Protein antibody [HL1033] (GTX635873) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Myelin basic protein antibody [HL1033] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635873

GTX635873 ICC/IF Image

Myelin basic Protein antibody [HL1033] detects Myelin basic Protein protein at oligodendrocyte by immunofluorescent analysis.
Sample: DIV10 rat cortical neuron-no AraC cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Myelin basic Protein stained by Myelin basic Protein antibody [HL1033] (GTX635873) diluted at 1:500.
Red: Tau, a marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Anti-Myelin basic protein antibody [HL1033] used in IHC (Paraffin sections) (IHC-P). GTX635873

GTX635873 IHC-P Image

Myelin basic Protein antibody [HL1033] detects Myelin basic Protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Myelin basic Protein stained by Myelin basic Protein antibody [HL1033] (GTX635873) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Myelin basic protein antibody [HL1033] used in IHC (Paraffin sections) (IHC-P). GTX635873

GTX635873 IHC-P Image

Myelin basic Protein antibody [HL1033] detects Myelin basic Protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Myelin basic Protein stained by Myelin basic Protein antibody [HL1033] (GTX635873) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Myelin basic protein antibody [HL1033] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635873

GTX635873 ICC/IF Image

Myelin basic Protein antibody [HL1033] detects Myelin basic Protein protein by immunofluorescent analysis.
Sample: DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Myelin basic Protein stained by Myelin basic Protein antibody [HL1033] (GTX635873) diluted at 1:250.
Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Myelin basic protein antibody [HL1033] used in Western Blot (WB). GTX635873

GTX635873 WB Image

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Myelin basic Protein antibody [HL1033] (GTX635873) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Myelin basic protein antibody [HL1033] used in IHC (Frozen sections) (IHC-Fr). GTX635873

GTX635873 IHC-Fr Image

Myelin basic protein antibody [HL1033] detects Myelin basic protein protein at cell membrane by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: Myelin basic protein stained by Myelin basic protein antibody [HL1033] (GTX635873) diluted at 1:100.
Blue: Hoechst 33342 staining.

Anti-Myelin basic protein antibody [HL1033] used in IHC (Frozen sections) (IHC-Fr). GTX635873

GTX635873 IHC-Fr Image

Myelin basic protein antibody [HL1033] detects Myelin basic protein protein at cell membrane by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: Myelin basic protein stained by Myelin basic protein antibody [HL1033] (GTX635873) diluted at 1:100.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations