Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Brain: A fibrillar staining pattern should be seen.
Negative Control
Colon: MBP staining should be completely absent.
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 150-250) of human MBP
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
myelin basic protein
Cellular Localization
Nucleus
Background
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]
Database
Research Area
![Anti-Myelin basic protein antibody [MSVA-390R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04435](/upload/website/prouct_img/small/GTX04435/GTX04435_20230728_IHC-P_72_23072722_957.webp "Anti-Myelin basic protein antibody [MSVA-390R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04435")
![Anti-Myelin basic protein antibody [MSVA-390R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04435](/upload/website/prouct_img/small/GTX04435/GTX04435_20230728_IHC-P_202_23072723_111.webp "Anti-Myelin basic protein antibody [MSVA-390R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04435")
![Anti-Myelin basic protein antibody [MSVA-390R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04435](/upload/website/prouct_img/small/GTX04435/GTX04435_20230728_IHC-P_332_23072723_951.webp "Anti-Myelin basic protein antibody [MSVA-390R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04435")
![Anti-Myelin basic protein antibody [MSVA-390R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04435](/upload/website/prouct_img/normal/GTX04435/GTX04435_20230728_IHC-P_72_23072722_957.webp "Anti-Myelin basic protein antibody [MSVA-390R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04435")
![Anti-Myelin basic protein antibody [MSVA-390R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04435](/upload/website/prouct_img/normal/GTX04435/GTX04435_20230728_IHC-P_202_23072723_111.webp "Anti-Myelin basic protein antibody [MSVA-390R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04435")
![Anti-Myelin basic protein antibody [MSVA-390R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04435](/upload/website/prouct_img/normal/GTX04435/GTX04435_20230728_IHC-P_332_23072723_951.webp "Anti-Myelin basic protein antibody [MSVA-390R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04435")