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Myelin basic protein antibody [rMBP/4288]

Anti-Myelin basic protein antibody [rMBP/4288] used in Western Blot (WB). GTX02673
Anti-Myelin basic protein antibody [rMBP/4288] used in IHC (Paraffin sections) (IHC-P). GTX02673
Anti-Myelin basic protein antibody [rMBP/4288] used in IHC (Paraffin sections) (IHC-P). GTX02673

Cat. No. GTX02673

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 rMBP/4288

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($529)
See all Myelin basic protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2-4 μg/ml
IHC-P 1-2 μg/ml

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Observed MW

14-22 kDa.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 150-250) of human MBP

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myelin basic protein

Background

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Myelin basic protein antibody [rMBP/4288] used in Western Blot (WB). GTX02673

GTX02673 WB Image

WB analysis of human brain tissue lysate using GTX02673 Myelin basic protein antibody [rMBP/4288].

Anti-Myelin basic protein antibody [rMBP/4288] used in IHC (Paraffin sections) (IHC-P). GTX02673

GTX02673 IHC-P Image

IHC-P analysis of human cerebellum tissue section using GTX02673 Myelin basic protein antibody [rMBP/4288].

Anti-Myelin basic protein antibody [rMBP/4288] used in IHC (Paraffin sections) (IHC-P). GTX02673

GTX02673 IHC-P Image

IHC-P analysis of human cerebellum tissue section using GTX02673 Myelin basic protein antibody [rMBP/4288].

REFERENCE

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REVIEW

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Package List Price ($)
$ 529