Myelin basic protein antibody

Name: Myelin basic protein antibody
Brand: GeneTex
SKU: GTX133108
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-Myelin basic protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108

Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

Anti-Myelin basic protein antibody used in Western Blot (WB). GTX133108

Cat. No. GTX133108

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IHC, IHC (Free Floating)
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX133108 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:10000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IHC	Assay dependent
IHC (Free Floating)	Assay dependent

Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Myelin Basic Protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886829

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myelin basic protein , C76307 , Hmbpr , R75289 , golli-mbp , jve , mld , shi

Cellular Localization

Isoform 13: Myelin membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 12: Myelin membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 11: Myelin membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 10: Myelin membr

Background

The protein encoded by the classic Mbp gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, Mbp-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long Mbp gene (otherwise called "Golli-Mbp") that contains 3 additional exons located upstream of the classic Mbp exons. Alternative splicing from the Golli and the Mbp transcription start sites gives rise to 2 sets of Mbp-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-Mbp, spliced in-frame to 1 or more Mbp exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to Mbp aa sequence. The second family of transcripts contain only Mbp exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the Mbp transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Mutation of the Mbp gene is associated with the 'shiverer' and 'myelin deficient' phenotypes in mouse. [provided by RefSeq, Jul 2008]

Database

Gene ID: 17196 Mbp
UniProt: P04370 Mbp

Research Area

DATA IMAGES

GTX133108 IHC-Fr Image

Myelin Basic Protein antibody detects Myelin Basic Protein protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Myelin Basic Protein protein stained by Myelin Basic Protein antibody (GTX133108) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133108 IHC-P Image

Myelin basic Protein antibody detects Myelin basic Protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Myelin basic Protein stained by Myelin basic Protein antibody (GTX133108) diluted at 1:1500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133108 IHC-P Image

Myelin Basic Protein antibody detects Myelin Basic Protein protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Myelin Basic Protein antibody (GTX133108) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133108 IHC-P Image

Myelin basic protein antibody detects Myelin basic protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Myelin basic protein stained by Myelin basic protein antibody (GTX133108) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133108 IHC-P Image

GTX133108 IHC-Fr Image

Myelin Basic Protein antibody detects Myelin Basic Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Myelin Basic Protein stained by Myelin Basic Protein antibody (GTX133108) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133108 IHC-Fr Image

Myelin Basic Protein antibody detects Myelin Basic protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: Myelin Basic protein stained by Myelin Basic Protein antibody (GTX133108) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

GTX133108 IHC-P Image

Myelin basic protein antibody detects Myelin basic protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Myelin basic protein stained by Myelin basic protein antibody (GTX133108) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133108 IHC-P Image

Myelin basic Protein antibody detects Myelin basic Protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Myelin basic Protein stained by Myelin basic Protein antibody (GTX133108) diluted at 1:1500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133108 WB Image

Mouse tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Myelin basic Protein antibody (GTX133108) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133108 IHC-P Image

GTX133108 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Myelin basic protein antibody (GTX133108) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

SH Chen et al. Neurotherapeutics 2024; 21 (3) : e00336 Ultrahigh frequency transcutaneous electrical nerve stimulation for neuropathic pain alleviation and neuromodulation

Application : IHC-Fr

Ruiz-Palacios M et al. Sci Total Environ 2020; 708 : 134821 Establishment of a brain cell line (FuB-1) from mummichog (Fundulus heteroclitus) and its application to fish virology, immunity and nanoplastics toxicology.

Application : ICC/IF

Doppler K et al. J Neurol Neurosurg Psychiatry 2018; (Epub) Neurofascin-155 IgM autoantibodies in patients with inflammatory neuropathies.

Application : IHC

Reactivity : Human

Oliveira CR et al. Dev Biol 2017; (Epub) Pseudotyped baculovirus is an effective gene expression tool for studying molecular function during axolotl limb regeneration.

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REVIEW