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Myelin basic protein antibody

Anti-Myelin basic protein antibody used in Western Blot (WB). GTX32733
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX32733
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX32733
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX32733

Cat. No. GTX32733

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Myelin basic protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human MBP (NP_001020271.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myelin basic protein

Background

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Myelin basic protein antibody used in Western Blot (WB). GTX32733

GTX32733 WB Image

WB analysis of various sample lysates using GTX32733 Myelin basic protein antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX32733

GTX32733 IHC-P Image

IHC-P analysis of human brain tissue using GTX32733 Myelin basic protein antibody.
Dilution : 1:100

Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX32733

GTX32733 IHC-P Image

IHC-P analysis of rat brain tissue using GTX32733 Myelin basic protein antibody.
Dilution : 1:100

Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX32733

GTX32733 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32733 Myelin basic protein antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399