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Myeloperoxidase antibody [2C7]

Cat No. GTX75315

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2C7

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, FACS, ELISA, IHC

Reactivity

 Human
Package
200 μg ($379)
See all Myeloperoxidase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1/500-1/1,000
IHC-Fr 1/1,000-1/5,000
FACS 1/50-1/100
ELISA Assay dependent
IHC Assay dependent

Note :

FACS
Membrane permeabilisation is required for this application.
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes recombinant MPO in Western blots and weakly in ELISA.Mouse anti Human Myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human myeloperoxidase.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Myeloperoxidase , Mpo

Background

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]

Database

Research Area

REFERENCE

Application Reference

Gardet A et al. J Immunol 2010; 185 (9) : 5577-85 LRRK2 is involved in the IFN-gamma response and host response to pathogens.
Application : IHC
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379