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Myeloperoxidase antibody [2C7]

Cat. No. GTX75315

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2C7

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, FACS, ELISA, IHC

Reactivity

 Human
Package
200 μg ($459)
See all Myeloperoxidase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1/500-1/1,000
IHC-Fr 1/1,000-1/5,000
FACS 1/50-1/100
ELISA Assay dependent
IHC Assay dependent

Note :

FACS
Membrane permeabilisation is required for this application.
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Product Note

This antibody recognizes recombinant MPO in Western blots and weakly in ELISA.Mouse anti Human Myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human myeloperoxidase.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_376717

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myeloperoxidase

Cellular Localization

Lysosome

Background

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]

Database

Research Area

REFERENCE

Clone Reference

Su M et al. Nat Cardiovasc Res 2022; 1 (8):732-747 Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis.
Kanakaraju Kaliannan et al. Int J Mol Sci 2022; 23 (10) Decreased Tissue Omega-6/Omega-3 Fatty Acid Ratio Prevents Chemotherapy-Induced Gastrointestinal Toxicity Associated with Alterations of Gut Microbiome.
Hasegawa J et al. Intern Med 2018; (Epub) An Autopsy Case of Myeloperoxidase-anti-neutrophil Cytoplasmic Antibody (MPO-ANCA) -associated Vasculitis Accompanied by Cryoglobulinemic Vasculitis Affecting the Kidneys, Skin, and Gastrointestinal Tract.
Futamata E et al. Nephron 2017; (Epub) Vanishing Immunoglobulins: The Formation of Pauci-Immune Lesions in Myeloperoxidase-Antineutrophil Cytoplasmic Antibody-Associated Vasculitis.
Rocha JD et al. Sci Rep 2015; 5:8008 Capsular polysaccharides from Cryptococcus neoformans modulate production of neutrophil extracellular traps (NETs) by human neutrophils.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459