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Myeloperoxidase antibody [2C7]

Anti-Myeloperoxidase antibody [2C7] used in Flow cytometry (FACS). GTX75318
Anti-Myeloperoxidase antibody [2C7] used in IHC (Paraffin sections) (IHC-P). GTX75318

Cat No. GTX75318

Host

Mouse

Clonality

Monoclonal

Clone Name

2C7

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA, IHC

Reactivity

Human
Package
40 μg ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1/500-1/1,000
IHC-Fr 1/1,000-1/5,000
FACS 1/50-1/100
ELISA Assay dependent
IHC Assay dependent

Note :

FACS
Membrane permeabilisation is required for this application.

Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

Bone marrow

Specificity/Sensitivity

GTX75318 recognises native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognises recombinant MPO in Western blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human myeloperoxidase.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Myeloperoxidase , Mpo

Background

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-Myeloperoxidase antibody [2C7] used in Flow cytometry (FACS). GTX75318

GTX75318 FACS Image

FACS analysis of human peripheral blood granulocytes using GTX75318 Myeloperoxidase antibody [2C7].

Anti-Myeloperoxidase antibody [2C7] used in IHC (Paraffin sections) (IHC-P). GTX75318

GTX75318 IHC-P Image

IHC-P analysis of human bone marrow tissue using GTX75318 Myeloperoxidase antibody [2C7].

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 169