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Myeloperoxidase antibody

Orthogonal
Anti-Myeloperoxidase antibody used in Western Blot (WB). GTX135125
Anti-Myeloperoxidase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135125

Cat. No. GTX135125

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Myeloperoxidase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Predict Reactivity

Cat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Myeloperoxidase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887450

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myeloperoxidase

Cellular Localization

Lysosome

Background

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-Myeloperoxidase antibody used in Western Blot (WB). GTX135125

GTX135125 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Myeloperoxidase antibody (GTX135125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Myeloperoxidase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135125

GTX135125 ICC/IF Image

Myeloperoxidase antibody detects Myeloperoxidase protein at vesicle by immunofluorescent analysis.
Sample: HL-60 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Myeloperoxidase stained by Myeloperoxidase antibody (GTX135125) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169