GeneTex
United States (US)

MyoD1 antibody [N1N3]

GTX100885_WB_19011506_119.jpg

Cat No. GTX100885

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ChIP assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , Raji , *CL1-0 , *CL1-5 ,

Predict Reactivity

Rat, Sheep, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MyoD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myogenic Differentiation 1,Myf3,Myod,Pum,Bhlhc1,Myod1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100885_WB_19011506_119.jpg

GTX100885 WB Image

Sample(30 µg of whole cell lysate)
A:293T
B:HeLa S3(GTX14654)
12% SDS PAGE
GTX100885 diluted at 1:200
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

MyoD1 antibody [N1N3] Cat No. GTX100885

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 18-Jul-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : CL1-0 cells,CL1-5 cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169