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MyoD1 antibody [SPM427]

Anti-MyoD1 antibody [SPM427] used in IHC (Paraffin sections) (IHC-P). GTX34882

Cat. No. GTX34882

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM427

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Chicken
Package
100 μg ($529)
See all MyoD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Product Note

Recognizes a phospho-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of Mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-MyoD1 antibody [SPM427] used in IHC (Paraffin sections) (IHC-P). GTX34882

GTX34882 IHC-P Image

IHC-P analysis of human rhabdomyosarcoma tissue using GTX34882 MyoD1 antibody [SPM427].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529