MyoD1 antibody [rMYD712]

Cat. No. GTX17751

Host	Mouse
Clonality	Monoclonal
Clone Name	rMYD712
Isotype	IgG1
Application	IHC-P, Protein Array
Reactivity	Human
Package	100 μg ($529)

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Datasheet
Document

View product citations for antibody GTX17751 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
IHC-P	1-2μg/ml for 30 minutes at RT
Protein Array	Assay dependent
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Product Note

Recognizes a phospho-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human MyoD1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYF3 , MYOD , MYOD1 , PUM , bHLHc1 , myogenic differentiation 1 , MyoD1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

Database

Gene ID: 4654 MYOD1
UniProt: P15172 MYOD1

Research Area

DATA IMAGES

GTX17751 IHC-P Image

IHC-P analysis of human rhabdomyosarcoma tissue using GTX17751 MyoD1 antibody [rMYD712].

GTX17751 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using MyoD1 Mouse Recombinant Monoclonal Antibody (rMYD712). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty