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MyoD1 antibody

Anti-MyoD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03495
Anti-MyoD1 antibody used in Western Blot (WB). GTX03495

Cat. No. GTX03495

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
400 μl ($399)
See all MyoD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:4000
ICC/IF 1:25
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human MyoD1.

Purification

Protein A purified followed by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myogenic differentiation 1 , MYF3 , MYOD , PUM , bHLHc1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MyoD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03495

GTX03495 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed HeLa cells using GTX03495 MyoD1 antibody.
Green : Primary antibody
Red : Actin
Permeabilization : 0.1% Triton X-100
Dilution : 1:25

Anti-MyoD1 antibody used in Western Blot (WB). GTX03495

GTX03495 WB Image

WB analysis of various sample lysates using GTX03495 MyoD1 antibody.
Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Human placenta tissue lysate
Lane 3 : RD whole cell lysate
Lane 4 : HL-60 whole cell lysate
Lane 5 : HeLa whole cell lysate
Lane 6 : HT-1080 whole cell lysate
Lane 7 : U-2OS whole cell lysate
Dilution : 1:4000
Loading : 20 μg per lane

REFERENCE

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Package List Price ($)
$ 399