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MyoD1 (phospho Ser200) antibody

Orthogonal
Anti-MyoD1 (phospho Ser200) antibody used in Western Blot (WB). GTX50144

Cat. No. GTX50144

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μl ($399)
See all MyoD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Predict Reactivity

Human, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from human MyoD1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myogenic differentiation 1 , MYF3 , MYOD , PUM , bHLHc1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MyoD1 (phospho Ser200) antibody used in Western Blot (WB). GTX50144

GTX50144 WB Image

Orthogonal

WB analysis of extracts from C2C12 and 3T3 cells untreated or treated with EGF using GTX50144 MyoD1 (phospho Ser200) antibody .

REFERENCE

Application Reference

Boimpoundi Eunice Flavie Ouali et al. Biochem Biophys Rep 2021; 26 : 101019 Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399