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Myogenin antibody [F5D]

Anti-Myogenin antibody [F5D] used in IHC (Paraffin sections) (IHC-P). GTX21835
Anti-Myogenin antibody [F5D] used in Western Blot (WB). GTX21835

Cat. No. GTX21835

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 F5D

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
500 μl ($439)
See all Myogenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:50‐1:100

Note :

IHC-P
Recommendations for antigen retrieval : EDTA buffer pH 8.0 at room temperature for 30 mins.
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Antigen Species

Rat

Immunogen

BALB/C mice were injected with recombinant protein containing rat myogenin amino acid 30-224.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_384701

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myogenin

Cellular Localization

Nucleus

Background

This gene belongs to the myogenic factor subfamily of the basic helix-loop-helix (BHLH) family of transcription factors. The protein encoded by this gene is a muscle-specific skeletal myogenin, which acts in concert with other family members to regulate myogenesis. The protein plays an essential role in the conversion of undifferentiated cells to myoblasts and functional skeletal muscle. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Myogenin antibody [F5D] used in IHC (Paraffin sections) (IHC-P). GTX21835

GTX21835 IHC-P Image

IHC-P analysis of human rhabdomyosarcoma tissue using GTX21835 Myogenin antibody [F5D].

Anti-Myogenin antibody [F5D] used in Western Blot (WB). GTX21835

GTX21835 WB Image

The data was published in the journal PLoS One in 2015.PMID: 25875253

REFERENCE

Application Reference

Nguyen NU et al. PLoS One 2015; 10 (4) : e0124762 Dual roles of palladin protein in in vitro myogenesis: inhibition of early induction but promotion of myotube maturation.
Application : WB
Reactivity : Mouse
Kaur K et al. Cell Reprogram 2014; 5-Azacytidine Promotes the Transdifferentiation of Cardiac Cells to Skeletal Myocytes.
Peck GR et al. J Biol Chem 2009; 284 (44) : 30016-23 *-stimulated phosphorylation of the rab gtpase-activating protein tbc1d1 regulates glut4 translocation.
Application : WB
Reactivity : Mouse
Nedachi T et al. Am J Physiol Endocrinol Metab 2006; 291 (4) : E817-28 regulation of glucose transporters by * and extracellular glucose in c2c12 myotubes.
Application : WB
Reactivity : Mouse

Clone Reference

Paola L Carvajal Monroy et al. PLoS One 2013; 8 (3) A rat model for muscle regeneration in the soft palate
Application : IHC-P
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 439