GeneTex
United States (US)

Myogenin antibody [F5D]

GTX34885_20170712_IHC-P_18121410_221.jpg

Cat No. GTX34885

Host Mouse
Clonality Monoclonal
Clone Name F5D
Isotype IgG1
Application IHC-P
Reactivity Human, Mouse, Rat, Cat, Dog, Pig
APPLICATION

Application Note

IHC-P:0.5-1.0ug/ml for 30 minutes at RT.

Calculated MW

33 kDa. ( Note )

Specificity/Sensitivity

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms� tumor.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Rat myogenin peptide (aa 73-94) followed by rat myogenin recombinant fragment (aa30-224) (Epitope aa138-158)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myogenin,Myog

Background

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES
GTX34885_20170712_IHC-P_18121410_221.jpg

GTX34885 IHC-P Image

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Myogenin Monoclonal Antibody (F5D)

Package List Price ($)
$ 329