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Myogenin antibody [F5D]

Anti-Myogenin antibody [F5D] used in IHC (Paraffin sections) (IHC-P). GTX34885

Cat. No. GTX34885

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 F5D

Isotype

 IgG1

Application

 IHC-P, ELISA

Reactivity

 Human, Mouse, Rat, Cat, Dog, Pig
Package
100 μg ($529)
See all Myogenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1-2μg/ml for 30 minutes at RT
ELISA Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat myogenin peptide (aa 73-94) followed by rat myogenin recombinant fragment (aa30-224) (Epitope aa138-158)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Nucleus

Background

This gene belongs to the myogenic factor subfamily of the basic helix-loop-helix (BHLH) family of transcription factors. The protein encoded by this gene is a muscle-specific skeletal myogenin, which acts in concert with other family members to regulate myogenesis. The protein plays an essential role in the conversion of undifferentiated cells to myoblasts and functional skeletal muscle. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Myogenin antibody [F5D] used in IHC (Paraffin sections) (IHC-P). GTX34885

GTX34885 IHC-P Image

IHC-P analysis of human rhabdomyosarcoma tissue using GTX34885 Myogenin antibody [F5D].

REFERENCE

Clone Reference

Nguyen NU et al. PLoS One 2015; 10 (4):e0124762 Dual roles of palladin protein in in vitro myogenesis: inhibition of early induction but promotion of myotube maturation.
Kaur K et al. Cell Reprogram 2014; 5-Azacytidine Promotes the Transdifferentiation of Cardiac Cells to Skeletal Myocytes.
Peck GR et al. J Biol Chem 2009; 284 (44):30016-23 *-stimulated phosphorylation of the rab gtpase-activating protein tbc1d1 regulates glut4 translocation.
Nedachi T et al. Am J Physiol Endocrinol Metab 2006; 291 (4):E817-28 regulation of glucose transporters by * and extracellular glucose in c2c12 myotubes.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529