Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Myogenin antibody [HL1913] (GTX637662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Myogenin antibody [HL1913] (GTX637662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Myogenin antibody [HL1913] (GTX637662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Myogenin antibody [HL1913] (GTX637662) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.