GeneTex
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Myogenin antibody [MGN185]

Anti-Myogenin antibody [MGN185] used in IHC (Paraffin sections) (IHC-P). GTX34884

Cat No. GTX34884

Host Mouse
Clonality Monoclonal
Clone Name MGN185
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Cat, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Specificity/Sensitivity

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms� tumor.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Human myogenin recombinant protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myogenin,Myf4,Bhlhc3,Myf-4,Myog

Background

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Myogenin antibody [MGN185] used in IHC (Paraffin sections) (IHC-P). GTX34884

GTX34884 IHC-P Image

Formalin-fixed, paraffin-embedded Rhabdomyosarcoma stained with Myogenin Monoclonal Antibody (MGN185)

Package List Price ($)
$ 329