GeneTex
United States (US)

Myogenin antibody [SPM144]

GTX34886_20170712_IHC-P_18121410_157.jpg

Cat No. GTX34886

Host Mouse
Clonality Monoclonal
Clone Name SPM144
Isotype IgG1
Application IHC-P
Reactivity Human, Mouse, Rat, Cat, Pig
APPLICATION

Application Note

IHC-P:0.5-1.0ug/ml for 30 minutes at RT.

Calculated MW

25 kDa. ( Note )

Specificity/Sensitivity

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms� tumor.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Human myogenin recombinant protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myogenin,Myf4,Bhlhc3,Myf-4,Myog

Background

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX34886_20170712_IHC-P_18121410_157.jpg

GTX34886 IHC-P Image

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Myogenin Monoclonal Antibody (SPM144)

Package List Price ($)
$ 329