This antibody may be diluted to a titer of 1:50-1:200 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.
This antibody reacts with human myoglobin. The antibody stains strongly with skeletal and cardiac muscles. No non-specific staining with other tissues has been observed.
This antibody contains sodium azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
4mg/ml(Please refer to the vial label for the specific concentration.)
Myoglobin isolated from human heart.
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Myoglobin is a small heme-containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1-3 hours after onset of the symptoms, reaching peak level within 8-12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.
GTX29536 IHC Image
IHC Image using GTX29536 - Detection of Myoglobin by IHC in Human Skeletal Muscle