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Myosin 1C antibody

Anti-Myosin 1C antibody used in Western Blot (WB). GTX125947
Anti-Myosin 1C antibody used in Western Blot (WB). GTX125947
Anti-Myosin 1C antibody used in IHC (Paraffin sections) (IHC-P). GTX125947

Cat No. GTX125947

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

A431 , MYO1C transfected 293T lysate

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Myosin 1C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myosin IC , MMI-beta , MMIb , NMI , myr2

Cellular Localization

Cytoplasm , Cell membrane , Cell projection , stereocilium membrane , Isoform 3: Nucleus , nucleoplasm , Nucleus , nucleolus , nuclear pore complex

Background

This gene encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. The related gene myosin IE has been referred to as myosin IC in the literature, but it is a distinct locus on chromosome 19. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Myosin 1C antibody used in Western Blot (WB). GTX125947

GTX125947 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: MYO1C transfected 293T lysate
5% SDS PAGE
GTX125947 diluted at 1:5000

Anti-Myosin 1C antibody used in Western Blot (WB). GTX125947

GTX125947 WB Image

Sample (30 ug of whole cell lysate)
A: A431
5% SDS PAGE
GTX125947 diluted at 1:1000

Anti-Myosin 1C antibody used in IHC (Paraffin sections) (IHC-P). GTX125947

GTX125947 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using Myosin 1C(GTX125947) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for Myosin 1C antibody (GTX125947). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169