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Myosin Heavy Chain 11 antibody [SMMS-1]

Cat. No. GTX01765

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SMMS-1

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human
Package
500 μl ($399)
See all Myosin Heavy Chain 11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:100-1:200

Note :

IHC-P
Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended.
Not tested in other applications.

Calculated MW

227 kDa. ( Note )

Positive Control

Transitional cell carcinoma

Product Note

SMMS‐1 is an antibody to smooth muscle myosin, heavy chain that reacts with human visceral and vascular smooth muscle cells. The antibody also reacts with human myoepithelial cells. It is very helpful in distinguishing between benign sclerosing breast lesions and infiltrating carcinomas in difficult cases since it strongly stains the myoepithelial layer in the benign lesions while it is negative in the infiltrating carcinomas.

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant diluted in Tris-HCl buffer, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Antigen Species

Human

Immunogen

Crude human uterus extract

Purification

unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin heavy chain 11 , AAT4 , FAA4 , SMHC , SMMHC

Background

The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Clone Reference

2020; Novel SRF-ICA1L Fusions in Cellular Myoid Neoplasms With Potential For Malignant Behavior
Reactivity : Human
2020; Comparison of the efficiency of transgelin, smooth muscle myosin, and CD31 antibodies for the assessment of vascular tumor invasion and free tumor deposits in gastric, pancreatic, and colorectal adenocarcinomas
Application : IHC-P
Reactivity : Human
2019; Ectopic hamartomatous thymoma in an immunocompromised male
Application : IHC-P
Reactivity : Human
2019; CRTC1-MAML2 fusion in mucoepidermoid carcinoma of the breast
Reactivity : Human
2018; Pro-myogenic and low-oxygen culture increases expression of contractile smooth muscle markers in human fibroblasts
Application : WB
Reactivity : Human
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Package List Price ($)
$ 399