Myosin Light Chain 2 (MLC-2v) antibody [AT3B2]

Cat. No. GTX57570

Host	Mouse
Clonality	Monoclonal
Clone Name	AT3B2
Isotype	IgG2b
Application	WB, ICC/IF, FACS
Reactivity	Human, Rat
Package	100 μl ($399)

See all Myosin Light Chain 2 (MLC-2v) products

Datasheet
Document

View product citations for antibody GTX57570 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:2000
ICC/IF	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT3B2 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MYL4 protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin light chain 2 , CMH10 , MLC2 , MLC2s/v , MYL2

Cellular Localization

Cytoplasm,Myofibril,Sarcomere,A band

Background

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Database

Gene ID: 4633 MYL2
UniProt: P10916 MYL2

Research Area

DATA IMAGES

GTX57570 ICC/IF Image

ICC/IF analysis of A431 cells using GTX57570 Myosin Light Chain 2 (MLC-2v) antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

GTX57570 WB Image

WB analysis of Rat heart tissue lysate using GTX57570 Myosin Light Chain 2 (MLC-2v) antibody.
Loading : 60 μg
Dilution : 1:1000

GTX57570 FACS Image

FACS analysis of A431 cells using GTX57570 Myosin Light Chain 2 (MLC-2v) antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty