GeneTex
United States (US)

Myosin Light Chain 2 (MLC-2v) antibody

GTX100714_39995_20190118_WB_R_heart_19022001_162.jpg
GTX100714_39995_20181207_IHC-P_R_19011519_720.jpg
GTX100714_39995_20181207_IHC-P_M_19011519_336.jpg
GTX100714_39995_20181207_IHC-P_M_1_19011519_857.jpg
GTX100714_39995_20151112_WB_M_heart_19011506_667.jpg

Cat No. GTX100714

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

Mouse heart

Specificity/Sensitivity

This antibody detects Myosin light chain 2, slow skeletal/ventricular muscle isoform (MLC-2v) protein.

Predict Reactivity

Human, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Myosin Light Chain 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myosin Light Chain 2,Cmh10,Mlc-2S/V,Mlc2,Myl2

Background

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX100714_39995_20190118_WB_R_heart_19022001_162.jpg

GTX100714 WB Image

Whole cell extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Myosin Light Chain 2 (MLC-2v) antibody (GTX100714) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100714_39995_20181207_IHC-P_R_19011519_720.jpg

GTX100714 IHC-P Image

Myosin Light Chain 2 (MLC-2v) antibody detects Myosin Light Chain 2 (MLC-2v) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Myosin Light Chain 2 (MLC-2v) stained by Myosin Light Chain 2 (MLC-2v) antibody (GTX100714) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100714_39995_20181207_IHC-P_M_19011519_336.jpg

GTX100714 IHC-P Image

Myosin Light Chain 2 (MLC-2v) antibody detects Myosin Light Chain 2 (MLC-2v) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Myosin Light Chain 2 (MLC-2v) stained by Myosin Light Chain 2 (MLC-2v) antibody (GTX100714) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100714_39995_20181207_IHC-P_M_1_19011519_857.jpg

GTX100714 IHC-P Image

Myosin Light Chain 2 (MLC-2v) antibody detects Myosin Light Chain 2 (MLC-2v) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Myosin Light Chain 2 (MLC-2v) stained by Myosin Light Chain 2 (MLC-2v) antibody (GTX100714) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100714_39995_20151112_WB_M_heart_19011506_667.jpg

GTX100714 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Myosin Light Chain 2 antibody (GTX100714) diluted at 1:20000.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169