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Myosin Light Chain 2 (MLC-2v) antibody

Anti-Myosin Light Chain 2 (MLC-2v) antibody used in Western Blot (WB). GTX100888
Anti-Myosin Light Chain 2 (MLC-2v) antibody used in Western Blot (WB). GTX100888

Cat. No. GTX100888

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Myosin Light Chain 2 (MLC-2v) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

human heart

Product Note

This antibody detects Myosin light chain 2, slow skeletal/ventricular muscle isoform (MLC-2v) protein.

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Myosin Light Chain 2 (MLC-2v). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10632192

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin light chain 2 , CMH10 , MLC-2s/v , MLC2

Background

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Myosin Light Chain 2 (MLC-2v) antibody used in Western Blot (WB). GTX100888

GTX100888 WB Image

Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Myosin Light Chain 2 antibody (GTX100888) diluted at 1:1000.

Anti-Myosin Light Chain 2 (MLC-2v) antibody used in Western Blot (WB). GTX100888

GTX100888 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Myosin Light Chain 2 (MLC-2v) antibody (GTX100888) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Song EH et al. J Cell Sci 2015; 128 (5) : 913-22 Acetylation of the RhoA GEF Net1A controls its subcellular localization and activity.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169