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Myosin Light Chain 2 (MLC-2v) (phospho Ser15) antibody [HL2891]

Recombinant antibody Orthogonal
Anti-Myosin Light Chain 2 (MLC-2v) (phospho Ser15) antibody [HL2891] used in Western Blot (WB). GTX640170
Anti-Myosin Light Chain 2 (MLC-2v) (phospho Ser15) antibody [HL2891] used in Western Blot (WB). GTX640170

Cat. No. GTX640170

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2891

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Myosin Light Chain 2 (MLC-2v) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser15 of human Myosin Light Chain 2 (MLC-2v). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin light chain 2 , CMH10 , MLC-2s/v , MLC2

Background

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Myosin Light Chain 2 (MLC-2v) (phospho Ser15) antibody [HL2891] used in Western Blot (WB). GTX640170

GTX640170 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Myosin Light Chain 2 (MLC-2v) (phospho Ser15) antibody [HL2891] (GTX640170) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Myosin Light Chain 2 (MLC-2v) (phospho Ser15) antibody [HL2891] used in Western Blot (WB). GTX640170

GTX640170 WB Image

Orthogonal

Untreated (–) and treated (+) mouse tissue extract (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Myosin Light Chain 2 (MLC-2v) (phospho Ser15) antibody [HL2891] (GTX640170) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
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Custom Conjugations