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Myosin Light Chain 2 antibody

Anti-Myosin Light Chain 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50495
Anti-Myosin Light Chain 2 antibody used in Western Blot (WB). GTX50495

Cat No. GTX50495

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Specificity/Sensitivity

This antibody detects Myosin light chain 2, slow skeletal/ventricular muscle isoform (MLC-2v) protein when phosphorylated at Tyr118.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.17~21 (A-T-S-N-V) derived from human Myosin Light Chain 2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myosin light chain 9 , LC20 , MLC-2C , MLC2 , MRLC1 , MYRL2

Background

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Myosin Light Chain 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50495

GTX50495 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50495 Myosin Light Chain 2 antibody.

Anti-Myosin Light Chain 2 antibody used in Western Blot (WB). GTX50495

GTX50495 WB Image

WB analysis of extracts from 293 and 3T3 cells using GTX50495 Myosin Light Chain 2 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf