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Myosin Light Chain 2 antibody

Anti-Myosin Light Chain 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85778
Anti-Myosin Light Chain 2 antibody used in Western Blot (WB). GTX85778

Cat No. GTX85778

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP, ELISA

Reactivity

Human, Mouse, Rat
Package
50 μg ($419)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 2.5 μg/ml
IP Assay dependent
ELISA 1:5000 - 1:20000

Note :

IHC-P
Heat induced antigen retrieval in pH 6.0 citrate buffer

Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate pH 7.2, 0.15 M NaCl, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide from human MYL9.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

, LC20 , MLC2 , MLC2C , MRLC1 , MYL9 , MYRL2 , myosin light chain 9 , Myosin Light Chain 2

Background

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Myosin Light Chain 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85778

GTX85778 IHC-P Image

IHC-P analysis of human smooth muscle tissue using GTX85778 Myosin Light Chain 2 antibody.
Antigen retrieval : Heat-induced antigen retrieval
Dilution : 2.5 μg/ml

Anti-Myosin Light Chain 2 antibody used in Western Blot (WB). GTX85778

GTX85778 WB Image

WB analysis of vascular myosin (rat aorta, lane 1) and cardiac myosin (mouse heart, lane2) using GTX85778 Myosin Light Chain 2 antibody.
Dilution : 1:600

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419