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Myosin Light Chain 2 (phospho Ser19) antibody

Cat No. GTX24720

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Chicken
Package
50 μl ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

chicken gizzard myosin II phosphorylated in vitro by PAK

Product Note

This antibody detects phosphorylated Ser19 Myosin Light Chain 2. The sequence around Ser19 is conserved among MYL9, MYL12A and MYL12B protein.

Predict Reactivity

Human(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS(without Mg2+ and Ca2+) pH7.3, 50% glycerol with 1.0 mg/mL BSA (IgG, protease free), 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Chicken

Immunogen

synthetic phosphopeptide derived from the region of chicken MLC that contains serine 19 (serine 20 including the initiating methionine). Human (100% homologous) has not been tested, but is expected to cross-react.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_380401

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myosin light chain 9 , LC20 , MLC-2C , MLC2 , MRLC1 , MYRL2

Background

Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues. Phosphorylation of serine 19 (the major phosphorylation site and the preferred site for MLCK) activates myosin motor activity under physiological conditions.

Database

Research Area

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429