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Myosin Light Chain 2 (phospho Ser19) antibody

Anti-Myosin Light Chain 2 (phospho Ser19) antibody used in Western Blot (WB). GTX50178
Anti-Myosin Light Chain 2 (phospho Ser19) antibody used in IHC (Paraffin sections) (IHC-P). GTX50178

Cat No. GTX50178

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Specificity/Sensitivity

This antibody detects Myosin light chain 2, smooth muscle isoform (MYL9, also known as LC20) that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation.

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 19 (A-T-S(p)-N-V) derived from human Myosin Light chain 2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myosin light chain 9 , LC20 , MLC-2C , MLC2 , MRLC1 , MYRL2

Background

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Myosin Light Chain 2 (phospho Ser19) antibody used in Western Blot (WB). GTX50178

GTX50178 WB Image

WB analysis of extracts from 293 cells untreated or treated with starvation using GTX50178 Myosin Light chain 2 (phospho Ser19) antibody.

Anti-Myosin Light Chain 2 (phospho Ser19) antibody used in IHC (Paraffin sections) (IHC-P). GTX50178

GTX50178 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50178 Myosin Light chain 2 (phospho Ser19) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf