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Myosin Light Chain 3 antibody [N1C3]

Anti-Myosin Light Chain 3 antibody [N1C3] used in Western Blot (WB). GTX102930
Anti-Myosin Light Chain 3 antibody [N1C3] used in Western Blot (WB). GTX102930

Cat. No. GTX102930

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Myosin Light Chain 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Mouse heart , Molt-4

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Myosin Light Chain 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950967

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin light chain 3 , CMH8 , MLC-lV/sb , MLC1SB , MLC1V , VLC1 , VLCl

Background

MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Myosin Light Chain 3 antibody [N1C3] used in Western Blot (WB). GTX102930

GTX102930 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
12% SDS PAGE
GTX102930 diluted at 1:1000
Anti-Myosin Light Chain 3 antibody [N1C3] used in Western Blot (WB). GTX102930

GTX102930 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
12% SDS PAGE
GTX102930 diluted at 1:10000

REFERENCE

Application Reference

Liu J et al. Stem Cells 2016; (Epub) CREG1 Interacts with Sec8 to Promote Cardiomyogenic Differentiation and Cell-Cell Adhesion.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169