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Myosin Light Chain 2 (phospho Ser19) antibody

Anti-Myosin Light Chain 2 (phospho Ser19) antibody used in IHC (Paraffin sections) (IHC-P). GTX22480
Anti-Myosin Light Chain 2 (phospho Ser19) antibody used in Western Blot (WB). GTX22480
Anti-Myosin Light Chain 2 (phospho Ser19) antibody used in Western Blot (WB). GTX22480

Cat No. GTX22480

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA

Reactivity

Human, Mouse
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
IHC-P 2.5 μg/ml
IHC-Fr Assay dependent
IP 1:100
ELISA 1:10000 - 1:30000
Not tested in other applications.

Positive Control

Cardiac myocytes

Product Note

This antibody detects phosphorylated Ser19 Myosin Light Chain 2. The sequence around Ser19 is conserved among MYL9, MYL12A and MYL12B protein.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to KLH - KKRPQRAT(pS)NVFAMFD (aa 12-27).

Purification

Purified by antigen-affinity chromatography.
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed agains

Conjugation

Unconjugated

RRID

AB_374325

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myosin light chain 12A , HEL-S-24 , MLC-2B , MLCB , MRCL3 , MRLC3 , MYL2B

Background

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. This antibody is specific for the phosphorylated form of myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.

Database

Research Area

DATA IMAGES

Anti-Myosin Light Chain 2 (phospho Ser19) antibody used in IHC (Paraffin sections) (IHC-P). GTX22480

GTX22480 IHC-P Image

Myosin Light chain (phospho Ser20) antibody (GTX22480) was used at 2.5 μg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Anti-Myosin Light Chain 2 (phospho Ser19) antibody used in Western Blot (WB). GTX22480

GTX22480 WB Image

Western blot of Myosin Light chain (phospho Ser20) antibody (GTX22480). Lane 1: Regulatory Light Chain Non-Phospho recombinant protein. Lane 2: Regulatory Light Chain Phospho recombinant protein. Lane 3: Smooth Muscle Non-Phospho recombinant protein. Lane 4: Smooth Muscle Phospho recombinant protein. Load: 50 ng per lane. Primary antibody at 1:1,000 overnight at 4ºC. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 60 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 20 kDa.

Anti-Myosin Light Chain 2 (phospho Ser19) antibody used in Western Blot (WB). GTX22480

GTX22480 WB Image

Myosin Light chain (phospho Ser20) antibody (GTX22480) was used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 ul of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amersham's substrate system

REFERENCE

REVIEW

Myosin Light Chain 2 (phospho Ser19) antibody Cat No. GTX22480

Rating ( Average 5 based on 2 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Neurosci in 2017.PMID: 28729440
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Development in 2015.PMID: 25715397
SDS
Sodium Azide.pdf
Package List Price ($)
$ 319
Currently in production