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Myosin skeletal fast antibody [MY-32]

Anti-Myosin skeletal fast antibody [MY-32] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17485
Anti-Myosin skeletal fast antibody [MY-32] used in IHC (Paraffin sections) (IHC-P). GTX17485
Anti-Myosin skeletal fast antibody [MY-32] used in Western Blot (WB). GTX17485

Cat. No. GTX17485

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MY-32

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine, Cat, Chicken, Guinea pig
Package
100 μl ($439)
See all Myosin skeletal fast products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Product Note

Monoclonal Anti-Skeletal Myosin is specific for the myosin heavy chain. It does not stain human or animal cardiac or smooth muscle myosin or cells grown by tissue culture (nonmuscle myosin). It has been demonstrated on human skeletal muscle that the antibody stains the fast twitch (type II) isomyosin molecules. Monoclonal Anti-Skeletal Myosin antibody does react with human rhabdomyosarcomas.

PROPERTIES

Form

Liquid

Buffer

Ascite

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rabbit muscle myosin

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin heavy chain 1 , myosin heavy chain 2 , HEL71 , MYHSA1 , MYHa , MyHC-2X/D , MyHC-2x , IBM3 , MYH2A , MYHSA2 , MYHas8 , MYPOP , MyHC-2A , MyHC-IIa

Database

Research Area

DATA IMAGES

Anti-Myosin skeletal fast antibody [MY-32] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17485

GTX17485 ICC/IF Image

A10 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500 Monoclonal Anti-Myosin (Skeletal, Fast), Clone: MY-32 . The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Anti-Myosin skeletal fast antibody [MY-32] used in IHC (Paraffin sections) (IHC-P). GTX17485

GTX17485 IHC-P Image

Staining of formalin-fixed, paraffin-embedded human skeletal muscle sections with Mouse Anti-Myosin (Skeletal, Fast) Clone: MY-32 diluted 1:400 using Goat Anti-Mouse IgG, FITC-conjugate.

Anti-Myosin skeletal fast antibody [MY-32] used in Western Blot (WB). GTX17485

GTX17485 WB Image

Cell and tissue lysates were separated on SDS-PAGE and probed with Mouse Anti-Myosin (Skeletal, Fast) Clone: MY-32 diluted to 1:750. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes1. H9C2; 2. A10; 3. C2C12; 4. Mouse Skeletal Muscle; 5. Rat Skeletal Muscle

REFERENCE

Application Reference

Ma M et al. Int J Mol Sci 2022; 23 (17) PPARGC1A Is a Moderator of Skeletal Muscle Development Regulated by miR-193b-3p.
Yu JA et al. Cell Death Discov 2021; 7 (1) : 73 LncRNA-FKBP1C regulates muscle fiber type switching by affecting the stability of MYH1B.
Application : IHC-P
Reactivity : Chicken
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 439