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Myotilin antibody

Anti-Myotilin antibody used in Western Blot (WB). GTX109905
Anti-Myotilin antibody used in IHC (Paraffin sections) (IHC-P). GTX109905
Anti-Myotilin antibody used in Western Blot (WB). GTX109905

Cat. No. GTX109905

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Myotilin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Myotilin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721019

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myotilin , LGMD1 , LGMD1A , MFM3 , TTID , TTOD

Cellular Localization

Cell membrane , sarcolemma , Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk

Background

This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Database

Research Area

DATA IMAGES

Anti-Myotilin antibody used in Western Blot (WB). GTX109905

GTX109905 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX109905 diluted at 1:3000

Anti-Myotilin antibody used in IHC (Paraffin sections) (IHC-P). GTX109905

GTX109905 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Myotilin(GTX109905) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Myotilin antibody used in Western Blot (WB). GTX109905

GTX109905 WB Image

The data was published in the journal PeerJ in 2018.PMID: 30065891

REFERENCE

Application Reference

Roberts MD et al. PeerJ 2018; 6 : e5338 Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169