GeneTex
United States (US)

Myotilin antibody

GTX54355_20180530_ICCIF_18121411_171.jpg
GTX54355_20180530_WB_18121411_551.jpg
GTX54355_20180530_IHC-P_18121411_770.jpg

Cat No. GTX54355

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

55 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 259-314 of human MYOT (NP_001129412.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myotilin,Lgmd1,Lgmd1A,Mfm3,Ttid,Ttod,Myot

Background

This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.[provided by RefSeq, Oct 2008]

Database

DATA IMAGES
GTX54355_20180530_ICCIF_18121411_171.jpg

GTX54355 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX54355 Myotilin antibody.
Blue : DAPI

GTX54355_20180530_WB_18121411_551.jpg

GTX54355 WB Image

WB analysis of various samples using GTX54355 Myotilin antibody.The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

GTX54355_20180530_IHC-P_18121411_770.jpg

GTX54355 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX54355 Myotilin antibody.
Dilution : 1:100

Package List Price ($)
$ 319