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N-Cadherin antibody [5D5]

Anti-N-Cadherin antibody [5D5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82992
Anti-N-Cadherin antibody [5D5] used in Flow cytometry (FACS). GTX82992
Anti-N-Cadherin antibody [5D5] used in Western Blot (WB). GTX82992
Anti-N-Cadherin antibody [5D5] used in IHC (Paraffin sections) (IHC-P). GTX82992
Anti-N-Cadherin antibody [5D5] used in IHC (Paraffin sections) (IHC-P). GTX82992

Cat. No. GTX82992

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5D5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all N-Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CDH2 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11164328

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 2 , CD325 , CDHN , CDw325 , NCAD

Cellular Localization

Cell membrane,Cell junction

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-N-Cadherin antibody [5D5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82992

GTX82992 ICC/IF Image

ICC/IF analysis of A431 cells using GTX82992 N-Cadherin antibody [5D5].
Green : N-Cadherin
Blue: DRAQ5 fluorescent DNA dye

Anti-N-Cadherin antibody [5D5] used in Flow cytometry (FACS). GTX82992

GTX82992 FACS Image

FACS analysis of PC-2 cells using GTX82992 N-Cadherin antibody [5D5].
Green : N-Cadherin
Purple : negative control

Anti-N-Cadherin antibody [5D5] used in Western Blot (WB). GTX82992

GTX82992 WB Image

WB analysis of A431 (1), NIH3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate using GTX82992 N-Cadherin antibody [5D5].

Anti-N-Cadherin antibody [5D5] used in IHC (Paraffin sections) (IHC-P). GTX82992

GTX82992 IHC-P Image

IHC-P analysis of human Placenta tissue using GTX82992 N-Cadherin antibody [5D5].

Anti-N-Cadherin antibody [5D5] used in IHC (Paraffin sections) (IHC-P). GTX82992

GTX82992 IHC-P Image

IHC-P analysis of human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D) using GTX82992 N-Cadherin antibody [5D5].

REFERENCE

Application Reference

Zeng B et al. Exp Cell Res 2019; 374 (2) : 365-373 LncRNA GAS5 suppresses proliferation, migration, invasion, and epithelial-mesenchymal transition in oral squamous cell carcinoma by regulating the miR-21/PTEN axis.
Application : WB
Reactivity : Human
Saydaminova K et al. Cancer Biol Ther 2016; : 43110 Sensitizing ovarian cancer cells to chemotherapy by interfering with pathways that are involved in the formation of cancer stem cells.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399