APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:50-1:200 |
| 1:20 |
| 5 μg |
Not tested in other applications.
Calculated MW
Product Note
This antibody is not suitable for frozen tissues in immunohistochemistry applications.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Bacterial fusion protein corresponding to the extracellular domain of N-cadherin.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cadherin 2 , CD325 , CDHN , CDw325 , NCAD
Cellular Localization
Cell membrane,Cell junction
Background
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
Database
Research Area
DATA IMAGES
![Anti-N-Cadherin antibody [8C11] used in IHC (Paraffin sections) (IHC-P). GTX19348](/templates/default/js/lightbox/images/loading.gif "Anti-N-Cadherin antibody [8C11] used in IHC (Paraffin sections) (IHC-P). GTX19348")
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GTX19348 IHC-P Image
IHC-P analysis of human heart tissue using GTX19348 N-Cadherin antibody [8C11].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
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GTX19348 WB Image
WB analysis of 25 ug of Hela (Lane 1), HUVEC (Lane 2), and C2C12 cell lysates (Lane 3) using GTX19348 N-Cadherin antibody [8C11].
Dilution : 1:1000 (Hela) and 1:500 (HUVEC and C2C12)
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GTX19348 ICC/IF Image
ICC/IF analysis of SH-SY5Y cells using GTX19348 N-Cadherin antibody [8C11]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature
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GTX19348 WB Image
WB analysis of 25 μg of various lysates using GTX19348 N-Cadherin antibody [8C11].
Dilution : 1:1000
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GTX19348 IHC-P Image
IHC-P analysis of human breast tissue using GTX19348 N-Cadherin antibody [8C11].
A : isotype control
B : Primary antibody
C : Primary antibody with magnified section
Antigen retrieval : HEIR with a buffer (pH 6.2)
Dilution : 2 μg/ml
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GTX19348 WB Image
WB analysis of tissue lysate and whole cell extract (30 ug) of Mouse Brain (Lane 1) and MCF-7 (Lane 2) using GTX19348 N-Cadherin antibody [8C11].
Dilution : 1:1000
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GTX19348 IP Image
IP analysis of SH-SY5Y cell lysates using GTX19348 N-Cadherin antibody [8C11].
GTX19348 was used as IP and IB antibody
IP reaction : 5μg antibody / 300μg lysate
WB antibody dilution : 1:1000
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REFERENCE
There are currently no references for N-Cadherin antibody [8C11] (GTX19348). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for N-Cadherin antibody [8C11] (GTX19348). Be the first to share your experience with this product.
![Anti-N-Cadherin antibody [8C11] used in IHC (Paraffin sections) (IHC-P). GTX19348](/upload/website/prouct_img/small/GTX19348/GTX19348_1069_IHC-P_w_23060620_202.webp "Anti-N-Cadherin antibody [8C11] used in IHC (Paraffin sections) (IHC-P). GTX19348")
![Anti-N-Cadherin antibody [8C11] used in Western Blot (WB). GTX19348](/upload/website/prouct_img/small/GTX19348/GTX19348_1566_WB_w_23060620_784.webp "Anti-N-Cadherin antibody [8C11] used in Western Blot (WB). GTX19348")
![Anti-N-Cadherin antibody [8C11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19348](/upload/website/prouct_img/small/GTX19348/GTX19348_364_ICC-IF_w_23060620_566.webp "Anti-N-Cadherin antibody [8C11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19348")
![Anti-N-Cadherin antibody [8C11] used in Western Blot (WB). GTX19348](/upload/website/prouct_img/small/GTX19348/GTX19348_1567_WB_w_23060620_176.webp "Anti-N-Cadherin antibody [8C11] used in Western Blot (WB). GTX19348")
![Anti-N-Cadherin antibody [8C11] used in IHC (Paraffin sections) (IHC-P). GTX19348](/upload/website/prouct_img/small/GTX19348/GTX19348_1068_IHC-P_w_23060620_515.webp "Anti-N-Cadherin antibody [8C11] used in IHC (Paraffin sections) (IHC-P). GTX19348")
![Anti-N-Cadherin antibody [8C11] used in Western Blot (WB). GTX19348](/upload/website/prouct_img/small/GTX19348/GTX19348_1568_WB_w_23060620_528.webp "Anti-N-Cadherin antibody [8C11] used in Western Blot (WB). GTX19348")
![Anti-N-Cadherin antibody [8C11] used in Immunoprecipitation (IP). GTX19348](/upload/website/prouct_img/small/GTX19348/GTX19348_1431_IP_w_23060620_760.webp "Anti-N-Cadherin antibody [8C11] used in Immunoprecipitation (IP). GTX19348")
![Anti-N-Cadherin antibody [8C11] used in IHC (Paraffin sections) (IHC-P). GTX19348](/upload/website/prouct_img/normal/GTX19348/GTX19348_1069_IHC-P_w_23060620_202.webp "Anti-N-Cadherin antibody [8C11] used in IHC (Paraffin sections) (IHC-P). GTX19348")
![Anti-N-Cadherin antibody [8C11] used in Western Blot (WB). GTX19348](/upload/website/prouct_img/normal/GTX19348/GTX19348_1566_WB_w_23060620_784.webp "Anti-N-Cadherin antibody [8C11] used in Western Blot (WB). GTX19348")
![Anti-N-Cadherin antibody [8C11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19348](/upload/website/prouct_img/normal/GTX19348/GTX19348_364_ICC-IF_w_23060620_566.webp "Anti-N-Cadherin antibody [8C11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19348")
![Anti-N-Cadherin antibody [8C11] used in Western Blot (WB). GTX19348](/upload/website/prouct_img/normal/GTX19348/GTX19348_1567_WB_w_23060620_176.webp "Anti-N-Cadherin antibody [8C11] used in Western Blot (WB). GTX19348")
![Anti-N-Cadherin antibody [8C11] used in IHC (Paraffin sections) (IHC-P). GTX19348](/upload/website/prouct_img/normal/GTX19348/GTX19348_1068_IHC-P_w_23060620_515.webp "Anti-N-Cadherin antibody [8C11] used in IHC (Paraffin sections) (IHC-P). GTX19348")
![Anti-N-Cadherin antibody [8C11] used in Western Blot (WB). GTX19348](/upload/website/prouct_img/normal/GTX19348/GTX19348_1568_WB_w_23060620_528.webp "Anti-N-Cadherin antibody [8C11] used in Western Blot (WB). GTX19348")
![Anti-N-Cadherin antibody [8C11] used in Immunoprecipitation (IP). GTX19348](/upload/website/prouct_img/normal/GTX19348/GTX19348_1431_IP_w_23060620_760.webp "Anti-N-Cadherin antibody [8C11] used in Immunoprecipitation (IP). GTX19348")