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N-Cadherin antibody [GC-4]

Cat. No. GTX11340

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GC-4

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr, FACS, EM

Reactivity

 Human, Mouse, Rat, Rabbit, Chicken
Package
100 μl ($469)
See all N-Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100
ICC/IF Assay dependent
IHC-Fr 1:100
FACS Assay dependent
EM Assay dependent
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

chicken or rat cardiac muscle

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Chicken

Immunogen

Affinity purified chicken heart A-CAM.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 2 , cadherin-2

Cellular Localization

Cell membrane

Background

Monoclonal Anti-N Cadherin/ACAM may be used for the localization of a 135 kDa receptor of the intercellular adherens junction in immunofluorescent microscopy, electron microscopy, and immunoblotting. This antibody can also be used to study the mechanism of adhesion and interaction of cultured cells and cells in tissues.Monoclonal Anti-NCadherin/ACAM stains a large variety of intercellular junctions formed by chicken fibroblastic and epithelial cells in culture. In adult mammals, the antibody stains predominantly heart and lens tissue and also stains a large variety of embryonic tissue. By double labelling, this antibody appears to localize vinculin and actin-rich intercellular contacts and is absent from cellular matrix attachment sites.

Database

Research Area

REFERENCE

Clone Reference

Kiel MJ et al. Cell Stem Cell 2007; 1 (2):204-17 Lack of evidence that hematopoietic stem cells depend on N-cadherin-mediated adhesion to osteoblasts for their maintenance.
Application : FACS
Reactivity : Mouse
Li G et al. Cancer Res 2001; 61 (9):700933 N-cadherin-mediated intercellular interactions promote survival and migration of melanoma cells.
Application : ICC/IF
Reactivity : Human
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 469