GeneTex
United States (US)

N-Cadherin antibody [N1N2], N-term

GTX101141_39549_20160520_IFA_19011506_645.jpg
GTX101141_39549_20160520_IFA_2_19011506_826.jpg
GTX101141_39549_WB_19011506_503.jpg
GTX101141_39549_20150925_IHC-Fr_M_2_19011506_773.jpg
GTX101141_39549_20150925_IHC-Fr_M_19011506_109.jpg

Cat No. GTX101141

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human N-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

neural-cadherin antibody, neural cadherin antibody, calcium-dependent adhesion protein, neuronal antibody, cadherin-2 antibody, cadherin 2, type 1, N-cadherin (neuronal) antibody, cadherin 2, N-cadherin (neuronal) antibody, NCAD antibody, N-cadherin 1 antibody, CDw325 antibody, CDHN antibody, CDH2 antibody, CD325 antibody

Cellular Localization

Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101141_39549_20160520_IFA_19011506_645.jpg

GTX101141 ICC/IF Image

N-Cadherin antibody [N1N2], N-term detects N-Cadherin protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: N-Cadherin protein stained by N-Cadherin antibody [N1N2], N-term (GTX101141) diluted at 1:400.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX101141_39549_20160520_IFA_2_19011506_826.jpg

GTX101141 ICC/IF Image

N-Cadherin antibody [N1N2], N-term detects N-Cadherin protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: N-Cadherin protein stained by N-Cadherin antibody [N1N2], N-term (GTX101141) diluted at 1:400.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX101141_39549_WB_19011506_503.jpg

GTX101141 WB Image

Sample (30 ug of whole cell lysate)
A: K562
5% SDS PAGE
GTX101141 diluted at 1:2000

GTX101141_39549_20150925_IHC-Fr_M_2_19011506_773.jpg

GTX101141 IHC-Fr Image

N-Cadherin antibody [N1N2] detects N-Cadherin protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody [N1N2] (GTX101141) diluted at 1:500.

GTX101141_39549_20150925_IHC-Fr_M_19011506_109.jpg

GTX101141 IHC-Fr Image

N-Cadherin antibody [N1N2] detects N-Cadherin protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: N-Cadherin antibody [N1N2](GTX101141) diluted at 1:250.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169