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N-Cadherin antibody [N1N3]

KO/KD Orthogonal
Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
Anti-N-Cadherin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112734
Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734
Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734
Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734
Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
Anti-N-Cadherin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112734
Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734

Cat. No. GTX112734

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all N-Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

PC-12 , Rat2 , rat brain , NT2D1

Predict Reactivity

Bovine, Dog, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human N-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729183

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 2 , CD325 , CDHN , CDw325 , NCAD

Cellular Localization

Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734

GTX112734 WB Image

KO/KD

Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112734

GTX112734 ICC/IF Image

N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: N-Cadherin stained by N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734

GTX112734 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734

GTX112734 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734

GTX112734 WB Image

N-Cadherin antibody [N1N3] detects N-Cadherin protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
B. 30 μg Rat2 whole cell extract
5% SDS-PAGE
N-Cadherin antibody [N1N3] (GTX112734) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734

GTX112734 IHC-P Image

N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
N-Cadherin stained by N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734

GTX112734 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-cadherin antibody (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734

GTX112734 IHC-P Image

N-Cadherin antibody [N1N3] detects CDH2 protein at membrane on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
N-Cadherin antibody [N1N3] (GTX112734) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734

GTX112734 IHC-P Image

N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
N-Cadherin stained by N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734

GTX112734 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112734

GTX112734 ICC/IF Image

Orthogonal

The data was published in the journal Oncotarget in 2016.PMID: 26840566

Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734

GTX112734 IHC-P Image

Orthogonal

The data was published in the journal Oncotarget in 2016.PMID: 26840566

REFERENCE

Application Reference

Meng-Hui Zhang et al. Bioengineered 2022; 13 (4) : 10047-10060 Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway.
Application : WB
Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Reactivity : Human
Ho KH et al. Pharmacol Res 2019; 147 : 104390 miR-140 targeting CTSB signaling suppresses the mesenchymal transition and enhances temozolomide cytotoxicity in glioblastoma multiforme.
Application : WB
Reactivity : Human
Chung IH et al. Oncotarget 2016; 7 (10) : 11113-26 Overexpression of lipocalin 2 in human cervical cancer enhances tumor invasion.
Application : IHC-P
Reactivity : Human
Jian MY et al. Am J Physiol Lung Cell Mol Physiol 2016; 310 (1) : L71-85 N-cadherin coordinates AMP kinase-mediated lung vascular repair.
Application : WB
Reactivity : Rat
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REVIEW

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Application Reference
Meng-Hui Zhang et al. Bioengineered 2022; 13 (4):10047-10060 Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway.
Application : WB
Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Reactivity : Human
Ho KH et al. Pharmacol Res 2019; 147:104390 miR-140 targeting CTSB signaling suppresses the mesenchymal transition and enhances temozolomide cytotoxicity in glioblastoma multiforme.
Application : WB
Reactivity : Human
Chung IH et al. Oncotarget 2016; 7 (10):11113-26 Overexpression of lipocalin 2 in human cervical cancer enhances tumor invasion.
Application : IHC-P
Reactivity : Human
Ling HH et al. Exp Cell Res 2016; Elevation of YAP promotes the epithelial-mesenchymal transition and tumor aggressiveness in colorectal cancer.
Application : WB
Reactivity : Human
Jian MY et al. Am J Physiol Lung Cell Mol Physiol 2016; 310 (1):L71-85 N-cadherin coordinates AMP kinase-mediated lung vascular repair.
Application : WB
Reactivity : Rat
Lupia A et al. J Invest Dermatol 2014; 134 (12):2947-56 CD63 tetraspanin is a negative driver of epithelial-to-mesenchymal transition in human melanoma cells.
Application : WB
Reactivity : Human
Hsu TH et al. Cell Death Differ 2014; Involvement of RARRES3 in the regulation of Wnt proteins acylation and signaling activities in human breast cancer cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169