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N-Cadherin antibody [N1N3]

Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
Anti-N-Cadherin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112734
Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734
Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734
Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734

Cat No. GTX112734

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

NT2D1 , U87-MG , SK-N-SH , IMR-32 , SK-N-AS , mouse brain , rat brain , PC-12 , Rat2

Predict Reactivity

Bovine, Dog, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human N-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729183

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 2 , CD325 , CDHN , CDw325 , NCAD

Cellular Localization

Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734

GTX112734 WB Image

Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112734

GTX112734 ICC/IF Image

N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: N-Cadherin stained by N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734

GTX112734 WB Image

N-Cadherin antibody [N1N3] detects N-Cadherin protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
B. 30 μg Rat2 whole cell extract
5% SDS-PAGE
N-Cadherin antibody [N1N3] (GTX112734) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734

GTX112734 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734

GTX112734 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734

GTX112734 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734

GTX112734 IHC-P Image

N-Cadherin antibody [N1N3] detects CDH2 protein at membrane on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
N-Cadherin antibody [N1N3] (GTX112734) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734

GTX112734 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-cadherin antibody (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734

GTX112734 IHC-P Image

N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
N-Cadherin stained by N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-N-Cadherin antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112734

GTX112734 IHC-P Image

N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
N-Cadherin stained by N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

N-Cadherin antibody [N1N3] Cat No. GTX112734

Rating ( Average 5 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 26840566
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 26840566
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf