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N-Cadherin antibody [N2C1], Internal

Anti-N-Cadherin antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX112733
Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733
Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733
Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733
Anti-N-Cadherin antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112733
Anti-N-Cadherin antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112733
Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733

Cat No. GTX112733

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

293T , NT2D1 , Mouse brain , Rat brain

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human N-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729640

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 2 , CD325 , CDHN , CDw325 , NCAD

Cellular Localization

Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-N-Cadherin antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX112733

GTX112733 IHC-Fr Image

N-Cadherin antibody [N2C1], Internal detects N-Cadherin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: N-Cadherin protein stained by N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733

GTX112733 WB Image

Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733

GTX112733 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:1000.

Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733

GTX112733 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:1000.

Anti-N-Cadherin antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112733

GTX112733 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using N-Cadherin(GTX112733) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-N-Cadherin antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112733

GTX112733 IHC-P Image

N-Cadherin antibody [N2C1], Internal detects CDH2 protein at membrane on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
N-Cadherin antibody [N2C1], Internal (GTX112733) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733

GTX112733 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

N-Cadherin antibody [N2C1], Internal Cat No. GTX112733

Rating ( Average 4 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 12-Jun-2019
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Neuroepithelial cells derived from human iPS cells
Blocking : 5% skim milk, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton X-100
Primary Antibody : 1:200, 4°C, 16Hr
Date : Anonymous submitted on 24-May-2019
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Neuroepithelial cells derived from human iPS cells
Blocking : 5% skim milk, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton X-100
Primary Antibody : 1:200, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf