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N-Cadherin antibody [N2C1], Internal

KO/KD
Anti-N-Cadherin antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX112733
Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733
Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733
Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733
Anti-N-Cadherin antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112733
Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733
Anti-N-Cadherin antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112733

Cat. No. GTX112733

Reference 6
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all N-Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

mouse brain , rat brain , NT2D1 , 293T

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human N-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729640

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 2 , CD325 , CDHN , CDw325 , NCAD

Cellular Localization

Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-N-Cadherin antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX112733

GTX112733 IHC-Fr Image

N-Cadherin antibody [N2C1], Internal detects N-Cadherin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: N-Cadherin protein stained by N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733

GTX112733 WB Image

KO/KD

Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733

GTX112733 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733

GTX112733 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:1000.

Anti-N-Cadherin antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112733

GTX112733 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using N-Cadherin(GTX112733) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-N-Cadherin antibody [N2C1], Internal used in Western Blot (WB). GTX112733

GTX112733 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:1000.

Anti-N-Cadherin antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112733

GTX112733 IHC-P Image

N-Cadherin antibody [N2C1], Internal detects CDH2 protein at membrane on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
N-Cadherin antibody [N2C1], Internal (GTX112733) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

? ?en et al. ACS Appl Mater Interfaces 2023; 15 (50) : 58260-58273 Microglia Polarization and Antiglioma Effects Fostered by Dual Cell Membrane-Coated Doxorubicin-Loaded Hexagonal Boron Nitride Nanoflakes
Liang ZL et al. J Appl Toxicol 2018; (Epub) Epiproteome profiling of cadmium-transformed human bronchial epithelial cells by quantitative histone post-translational modification-enzyme-linked immunosorbent assay.
Application : WB
Reactivity : Human
Chen YJ et al. PLoS One 2017; 12 (8) : e0183368 Novel histone deacetylase inhibitor AR-42 exhibits antitumor activity in pancreatic cancer cells by affecting multiple biochemical pathways.
Application : WB
Reactivity : Human
Wang TH et al. Oncotarget 2015; 6 (27) : 23342-57 Long non-coding RNA AOC4P suppresses hepatocellular carcinoma metastasis by enhancing vimentin degradation and inhibiting epithelial-mesenchymal transition.
Application : WB
Reactivity : Human
Chen CY et al. Oncotarget 2015; 6 (29) : 26946-59 Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.
Application : WB
Reactivity : Human
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REVIEW

N-Cadherin antibody [N2C1], Internal Cat. No. GTX112733

Rating
( Average 4 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 2 users reviews)
Anti-N-Cadherin antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112733
Date : Anonymous submitted on 12-Jun-2019
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Neuroepithelial cells derived from human iPS cells
Blocking : 5% skim milk, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton X-100
Primary Antibody : 1:200, 4°C, 16Hr
Anti-N-Cadherin antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112733
Date : Anonymous submitted on 24-May-2019
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Neuroepithelial cells derived from human iPS cells
Blocking : 5% skim milk, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton X-100
Primary Antibody : 1:200, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169