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N-Cadherin antibody

KO/KD Orthogonal
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127345
Anti-N-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX127345

Cat No. GTX127345

Reference 51
6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all N-Cadherin products
PRODUCT

Summary

N-Cadherin antibody recognizes N-cadherin protein, a calcium-dependent cell adhesion protein (predicted molecular weight of 100 kDa) encoded by the CDH2 gene. N-cadherin is involved in multiple processes, including neuron development and epithelial-mesenchymal transition (EMT). Due to the prominent role of EMT in cancer progression and metastasis, antibodies targeting either N-cadherin or E-cadherin are frequently used together to study the EMT process: the upregulation of N-cadherin followed by the downregulation of E-cadherin.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

293T , NT2D1 , SK-N-SH , mouse brain , rat brain , *A549

Predict Reactivity

Bovine, Dog, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human N-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885644

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 2 , CD325 , CDHN , CDw325 , NCAD

Cellular Localization

Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

KO/KD

Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345

GTX127345 IHC-Fr Image

N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345

GTX127345 IHC-Fr Image

N-Cadherin antibody detects N-Cadherin protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody (GTX127345) diluted at 1:500.

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127345

GTX127345 ICC/IF Image

N-Cadherin antibody detects N-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-N-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX127345

GTX127345 IHC-P Image

N-Cadherin antibody detects N-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
N-Cadherin stained by N-Cadherin antibody (GTX127345) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Lai YW et al. Cells 2021; 10 (8) Prognostic Value of a Glycolytic Signature and Its Regulation by Y-Box-Binding Protein 1 in Triple-Negative Breast Cancer.
Application : WB
Reactivity : Human
Wang LT et al. Cancer Lett 2021; 520 : 160-171 Phosphorylation of intestine-specific homeobox by ERK1 modulates oncogenic activity and sorafenib resistance.
Application : WB
Reactivity : Mouse
Belvedere R et al. Eur J Pharm Sci 2021; 163 : 105886 The promising pro-healing role of the association of mesoglycan and lactoferrin on skin lesions.
Milani M et al. J Neuroinflammation 2021; 18 (1) : 132 Targeting S100A4 with niclosamide attenuates inflammatory and profibrotic pathways in models of amyotrophic lateral sclerosis.
Application : WB
Reactivity : Human
Nicolai A et al. Pharmaceuticals (Basel) 2021; 14 (6) Anti-Tumoral Effects of a (1H-Pyrrol-1-yl)Methyl-1H-Benzoimidazole Carbamate Ester Derivative on Head and Neck Squamous Carcinoma Cell Lines.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

N-Cadherin antibody Cat No. GTX127345

Rating
( Average 4.8 based on 6 users reviews)
Application
Western Blot(WB)
( Average 4.8 based on 6 users reviews)
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal EMBO Rep in 2020.PMID: 31908141
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal EMBO Rep in 2020.PMID: 31908141
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal EMBO Rep in 2020.PMID: 31908141
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Date : Anonymous submitted on 26-Nov-2019
Score :
Application Tested : WB
Sample Species : Rat
Sample : Membranes from cortical neurons
Amount used : 50 μg
Blocking : 5% milk, 24°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Date : Anonymous submitted on 29-Dec-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : FaDu xenograft
Amount used : 30 μg
Blocking : 4% non-fat milk in TBST, 23°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Date : Anonymous submitted on 05-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 20μg
Blocking : 5% skim milk in TBST, 24°C, 1Hr
Primary Antibody : 1:3000, 24°C, 15Hr
Show More +
Submit a Review
Application Reference
Lai YW et al. Cells 2021; 10 (8) Prognostic Value of a Glycolytic Signature and Its Regulation by Y-Box-Binding Protein 1 in Triple-Negative Breast Cancer.
Application : WB
Reactivity : Human
Wang LT et al. Cancer Lett 2021; 520:160-171 Phosphorylation of intestine-specific homeobox by ERK1 modulates oncogenic activity and sorafenib resistance.
Application : WB
Reactivity : Mouse
Belvedere R et al. Eur J Pharm Sci 2021; 163:105886 The promising pro-healing role of the association of mesoglycan and lactoferrin on skin lesions.
Milani M et al. J Neuroinflammation 2021; 18 (1):132 Targeting S100A4 with niclosamide attenuates inflammatory and profibrotic pathways in models of amyotrophic lateral sclerosis.
Application : WB
Reactivity : Human
Nicolai A et al. Pharmaceuticals (Basel) 2021; 14 (6) Anti-Tumoral Effects of a (1H-Pyrrol-1-yl)Methyl-1H-Benzoimidazole Carbamate Ester Derivative on Head and Neck Squamous Carcinoma Cell Lines.
Application : WB
Reactivity : Human
Ou M et al. Oncol Rep 2021; 46 (1) MDM2 induces EMT via the B?Raf signaling pathway through 14?3?3.
Application : WB, IHC-P
Reactivity : Human
Li Y et al. Transl Lung Cancer Res 2021; 10 (4):1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.
Application : WB
Reactivity : Human
Milani M et al. Research Square 2021; (Epub) Niclosamide Targets Inammatory and Probrotic Pathways in Amyotrophic Lateral Sclerosis.
Application : WB
Hu W et al. JCI Insight 2021; 6 (3) NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.
Application : WB
Reactivity : Mouse
Chen YT et al. J Clin Invest 2021; 131 (5) Endoplasmic reticulum protein TXNDC5 promotes renal fibrosis by enforcing TGF-β signaling in kidney fibroblasts.
Application : WB
Reactivity : Mouse
Chen H.Y. et al. Cancers 2021; 13 (6):1236 Isoliquiritigenin Reverses Epithelial-Mesenchymal Transition Through Modulation of the TGF-β/Smad Signaling Pathway in Endometrial Cancer.
Application : WB, ICC/IF
Reactivity : Human
Duan C et al. Oncol Lett 2021; 21 (2):143 L-fucose ameliorates the carcinogenic properties of Fusobacterium nucleatum in colorectal cancer.
Application : WB
Reactivity : Human
Krzysiek-Maczka G et al. Microorganisms 2020; 8 (10) Long-Term Helicobacter pylori Infection Switches Gastric Epithelium Reprogramming Towards Cancer Stem Cell-Related Differentiation Program in Hp-Activated Gastric Fibroblast-TGFβ Dependent Manner.
Kuo TT et al. ACS Omega 2020; 5 (49):31792-31800 Melissa officinalis Extract Induces Apoptosis and Inhibits Migration in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Kuo CH et al. Cell Biochem Funct 2020; (Epub) Biomarker discovery in highly invasive lung cancer cell through proteomics approaches.
Application : WB
Reactivity : Human
Krzysiek-Maczka G et al. Microorganisms 2020; 8 (10) Long-Term Helicobacter pylori Infection Switches Gastric Epithelium Reprogramming Towards Cancer Stem Cell-Related Differentiation Program in Hp-Activated Gastric Fibroblast-TGFβ Dependent Manner.
Application : WB
Reactivity : Rat
Ho KH et al. Cell Mol Neurobiol 2020; miR-4286 is Involved in Connections Between IGF-1 and TGF-β Signaling for the Mesenchymal Transition and Invasion by Glioblastomas.
Application : WB
Reactivity : Human
Wang LT et al. EMBO Rep 2020; 21 (2):e48795 PCAF-mediated acetylation of ISX recruits BRD4 to promote epithelial-mesenchymal transition.
Lee JY et al. PLoS One 2020; 15 (7):e0236876 PHRF1 promotes migration and invasion by modulating ZEB1 expression.
Application : WB
Reactivity : Human
Wang SC et al. Biochem Pharmacol 2020; 180:114152 α-Linolenic acid inhibits the migration of human triple-negative breast cancer cells by attenuating Twist1 expression and suppressing Twist1-mediated epithelial-mesenchymal transition.
Application : WB
Reactivity : Human
Li Z et al. Front Cell Neurosci 2020; 14:144 MicroRNA-124 Overexpression in Schwann Cells Promotes Schwann Cell-Astrocyte Integration and Inhibits Glial Scar Formation Ability.
Application : WB
Reactivity : Rat
Le Saux G et al. ACS Appl Mater Interfaces 2020; 12 (20):22399-22409 Cell-Cell Adhesion-Driven Contact Guidance and Its Effect on Human Mesenchymal Stem Cell Differentiation.
Reactivity : Human
Fang CH et al. J Biomed Sci 2020; 27 (1):42 A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and 帣-catenin-ABCG2 signaling.
Application : WB
Reactivity : Human
Yang P et al. Int J Med Sci 2019; 16 (12):1631-1641 Agonist of PPAR-γ Reduced Epithelial-Mesenchymal Transition in Eosinophilic Chronic Rhinosinusitis with Nasal Polyps via Inhibition of High Mobility Group Box1.
Marcinkiewcz CA et al. ACS Chem Neurosci 2019; 10 (7):3154-3166 Sex-Dependent Modulation of Anxiety and Fear by 5-HT1A Receptors in the Bed Nucleus of the Stria Terminalis.
Raineri A et al. Int J Mol Sci 2019; 20 (23) Onconase Restores Cytotoxicity in Dabrafenib-Resistant A375 Human Melanoma Cells and Affects Cell Migration, Invasion and Colony Formation Capability.
Hsu SY et al. Onco Targets Ther 2019; 12:7611-7621 A novel CXCL8 analog is effective in inhibiting the growth via cell cycle arrest and attenuating invasion of Lewis lung carcinoma.
Application : WB
Reactivity : Mouse
Li-Chien Chang et al. Nutrition 2019; (Epub) 1,25(OH)2D3 attenuates indoxyl sulfate-induced epithelial-to-mesenchymal cell transition via inactivation of PI3K/Akt/?]-catenin signaling in renal tubular epithelial cells.
Kuo YL et al. Sci Rep 2019; 9 (1):10269 Hypoxia-induced epithelial-mesenchymal transition and fibrosis for the development of breast capsular contracture.
Application : WB
Reactivity : Mouse
Hung CY et al. Mol Neurobiol 2019; Sp1 in Astrocyte Is Important for Neurite Outgrowth and Synaptogenesis.
Application : WB
Reactivity : Mouse
Lee DK et al. Biochem Biophys Res Commun 2019; 514 (3):645-652 Cdk5 regulates N-cadherin-dependent neuronal migration during cortical development.
Reactivity : Mouse
Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
Application : WB
Reactivity : Human
Mansel C et al. Neurotoxicol Teratol 2019; 72:58-70 Lead exposure reduces survival, neuronal determination, and differentiation of P19 stem cells.
Application : ICC/IF
Reactivity : Mouse
Chang CY et al. Cell Death Discov 2018; 4:100 PEGylated liposome-encapsulated rhenium-188 radiopharmaceutical inhibits proliferation and epithelial-mesenchymal transition of human head and neck cancer cells in vivo with repeated therapy.
Application : WB
Reactivity : Mouse
DaSilva-Arnold S et al. Mol Hum Reprod 2018; (Epub) heZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation.
Application : WB
Reactivity : Human
Chen CM et al. J Renin Angiotensin Aldosterone Syst 2018; 19 (3):1.47E+15 Hyperglycemia activates the renin-angiotensin system and induces epithelial-mesenchymal transition in streptozotocin-induced diabetic kidneys.
Application : IHC-P
Li JM et al. Ther Adv Med Oncol 2018; 10:1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
Application : WB
Reactivity : Human
Hseu YC et al. J Cell Physiol 2018; 234 (4):4125-4139 Antrodia camphorata inhibits epithelial-to-mesenchymal transition by targeting multiple pathways in triple-negative breast cancers.
Application : WB
Reactivity : Human
Terasaki M et al. Anticancer Res 2018; 38 (4):2169-2179 Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.
Application : WB
Reactivity : Human
Chen CY et al. Int J Mol Sci 2018; 19 (2) Corylin Suppresses Hepatocellular Carcinoma Progression via the Inhibition of Epithelial-Mesenchymal Transition, Mediated by Long Noncoding RNA GAS5.
Application : WB
Reactivity : Human
Shentu TP et al. Sci Rep 2017; 7 (1):18052 Thy-1 dependent uptake of mesenchymal stem cell-derived extracellular vesicles blocks myofibroblastic differentiation.
Application : WB
Reactivity : Human
Chen PC et al. J Cell Physiol 2017; (Epub) Activation of Fibroblasts by Nicotine Promotes the Epithelial-Mesenchymal Transition and Motility of Breast Cancer Cells.
Application : WB
Reactivity : Human
Taglieri L et al. Invest New Drugs 2017; (Epub) The kinesin Eg5 inhibitor K858 induces apoptosis and reverses the malignant invasive phenotype in human glioblastoma cells.
Application : WB
Reactivity : Human
Chou HC et al. Toxicological Sciences 2017; (Epub) L-carnitine via PPARγ- and Sirt1-dependent mechanisms attenuates epithelial–mesenchymal transition and renal fibrosis caused by perfluorooctanesulfonate.
Application : WB
Chan SM et al. Sci Rep 2017; 7 (1):8646 Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells.
Application : WB
Reactivity : Human
Fujiki K et al. J Biol Chem 2017; (Epub) Involvement of Notch1 signaling in malignant progression of A549 cells subjected to prolonged cadmium exposure.
Application : WB
Reactivity : Human
Tong D et al. Cancer Lett 2016; 389:23-32 * inhibits castration-induced emt in prostate cancer by repressing cox2/pge2/stat3 axis.
Application : WB, IHC-P
Reactivity : Human
Jian MY et al. Am J Physiol Lung Cell Mol Physiol 2016; 310 (1):L71-85 N-cadherin coordinates AMP kinase-mediated lung vascular repair.
Application : WB
Reactivity : Rat
Li CH et al. Arch Toxicol 2016; (Epub) Cytoplasmic aryl hydrocarbon receptor regulates glycogen synthase kinase 3 beta, accelerates vimentin degradation, and suppresses epithelial-mesenchymal transition in non-small cell lung cancer cells.
Application : WB
Reactivity : Human
Jheng HF et al. Mol Nutr Food Res 2016; (Epub) Dietary low-fat soy milk powder retards diabetic nephropathy progression via inhibition of renal fibrosis and renal inflammation.
Application : IHC-P
Reactivity : Mouse
Chen LH et al. BMC Cancer 2016; 16:431 Involvement of DNMT 3B promotes epithelial-mesenchymal transition and gene expression profile of invasive head and neck squamous cell carcinomas cell lines.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169