GeneTex
  • Country / Location Selection

United States (US)

N-Cadherin antibody

Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX127345
Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

Cat No. GTX127345

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

293T , NT2D1 , SK-N-SH , mouse brain , rat brain , *A549

Predict Reactivity

Bovine, Dog, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human N-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 2 , CD325 , CDHN , CDw325 , NCAD

Cellular Localization

Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345

GTX127345 IHC-Fr Image

N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-N-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX127345

GTX127345 IHC-P Image

N-Cadherin antibody detects N-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
N-Cadherin stained by N-Cadherin antibody (GTX127345) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345

GTX127345 IHC-Fr Image

N-Cadherin antibody detects N-Cadherin protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody (GTX127345) diluted at 1:500.

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127345

GTX127345 ICC/IF Image

N-Cadherin antibody detects N-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

N-Cadherin antibody Cat No. GTX127345

Rating ( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4.7 based on 3 users reviews)
Date : Anonymous submitted on 26-Nov-2019
Score :
Application Tested : WB
Sample Species : Rat
Sample : Membranes from cortical neurons
Amount used : 50 μg
Blocking : 5% milk, 24°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 29-Dec-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : FaDu xenograft
Amount used : 30 μg
Blocking : 4% non-fat milk in TBST, 23°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 05-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 20μg
Blocking : 5% skim milk in TBST, 24°C, 1Hr
Primary Antibody : 1:3000, 24°C, 15Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169