GeneTex
United States (US)

N-Cadherin antibody

KO/KD
GTX127345_40975_20160921_IHC-Fr_18121410_944.jpg
GTX127345_40975_20180810_WB_KO_watermark_18121410_760.jpg
GTX127345_40975_20150925_IHC-Fr_M_18121410_823.jpg
GTX127345_40975_20180316_WB_18121410_160.jpg
GTX127345_40975_20160629_IFA_18121410_813.jpg
GTX127345_40975_20151105_WB_R_brain_18121410_982.jpg
GTX127345_40975_20151105_WB_M_brain_18121410_513.jpg
GTX127345_40975_IHC_18121410_106.jpg
GTX127345_40975_20180316_WB_2_18121410_461.jpg

Cat No. GTX127345

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

293T , NT2D1 , SK-N-SH , mouse brain , rat brain , *A549

Predict Reactivity

Bovine, Dog, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human N-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

neural-cadherin antibody, neural cadherin antibody, calcium-dependent adhesion protein, neuronal antibody, cadherin-2 antibody, cadherin 2, type 1, N-cadherin (neuronal) antibody, cadherin 2, N-cadherin (neuronal) antibody, NCAD antibody, N-cadherin 1 antibody, CDw325 antibody, CDHN antibody, CDH2 antibody, CD325 antibody

Cellular Localization

Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX127345_40975_20160921_IHC-Fr_18121410_944.jpg

GTX127345 IHC-Fr Image

N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX127345_40975_20180810_WB_KO_watermark_18121410_760.jpg

GTX127345 WB Image

KO/KD

Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127345_40975_20150925_IHC-Fr_M_18121410_823.jpg

GTX127345 IHC-Fr Image

N-Cadherin antibody detects N-Cadherin protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody (GTX127345) diluted at 1:500.

GTX127345_40975_20180316_WB_18121410_160.jpg

GTX127345 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127345_40975_20160629_IFA_18121410_813.jpg

GTX127345 ICC/IF Image

N-Cadherin antibody detects N-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX127345_40975_20151105_WB_R_brain_18121410_982.jpg

GTX127345 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127345_40975_20151105_WB_M_brain_18121410_513.jpg

GTX127345 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127345_40975_IHC_18121410_106.jpg

GTX127345 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using N-Cadherin antibody(GTX127345) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX127345_40975_20180316_WB_2_18121410_461.jpg

GTX127345 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Reference
REVIEW

N-Cadherin antibody Cat No. GTX127345

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 29-Dec-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : FaDu xenograft
Amount used : 30 μg
Blocking : 4% non-fat milk in TBST, 23°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 05-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 20μg
Blocking : 5% skim milk in TBST, 24°C, 1Hr
Primary Antibody : 1:3000, 24°C, 15Hr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf