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N-Cadherin antibody

KO/KD Orthogonal
Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Anti-N-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX127345

Cat No. GTX127345

Reference 24
2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
See all N-Cadherin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

293T , NT2D1 , SK-N-SH , mouse brain , rat brain , *A549

Predict Reactivity

Bovine, Dog, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human N-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cadherin 2,CD325,CDHN,CDw325,NCAD

Cellular Localization

Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345

GTX127345 IHC-Fr Image

N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

KO/KD

Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Orthogonal

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Anti-N-Cadherin antibody used in IHC (Frozen sections) (IHC-Fr). GTX127345

GTX127345 IHC-Fr Image

N-Cadherin antibody detects N-Cadherin protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody (GTX127345) diluted at 1:500.
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-N-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127345

GTX127345 ICC/IF Image

N-Cadherin antibody detects N-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345

GTX127345 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-N-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX127345

GTX127345 IHC-P Image

N-Cadherin antibody detects N-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
N-Cadherin stained by N-Cadherin antibody (GTX127345) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
REFERENCE

Application Reference

Li-Chien Chang et al. Nutrition 2019; (Epub) 1,25(OH)2D3 attenuates indoxyl sulfate-induced epithelial-to-mesenchymal cell transition via inactivation of PI3K/Akt/?]-catenin signaling in renal tubular epithelial cells.
Kuo YL et al. Sci Rep 2019; 9 (1) : 10269 Hypoxia-induced epithelial-mesenchymal transition and fibrosis for the development of breast capsular contracture.
Application : WB
Reactivity : Mouse
Hung CY et al. Mol Neurobiol 2019; Sp1 in Astrocyte Is Important for Neurite Outgrowth and Synaptogenesis.
Application : WB
Reactivity : Mouse
Lee DK et al. Biochem Biophys Res Commun 2019; 514 (3) : 645-652 Cdk5 regulates N-cadherin-dependent neuronal migration during cortical development.
Reactivity : Mouse
Mansel C et al. Neurotoxicol Teratol 2019; 72 : 58-70 Lead exposure reduces survival, neuronal determination, and differentiation of P19 stem cells.
Application : ICC/IF
Reactivity : Mouse
Show More +
Submit a Reference
REVIEW

N-Cadherin antibody Cat No. GTX127345

Rating
( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Date : Anonymous submitted on 29-Dec-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : FaDu xenograft
Amount used : 30 μg
Blocking : 4% non-fat milk in TBST, 23°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Anti-N-Cadherin antibody used in Western Blot (WB). GTX127345
Date : Anonymous submitted on 05-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 20μg
Blocking : 5% skim milk in TBST, 24°C, 1Hr
Primary Antibody : 1:3000, 24°C, 15Hr
Submit a Review
Application Reference
Li-Chien Chang et al. Nutrition 2019; (Epub) 1,25(OH)2D3 attenuates indoxyl sulfate-induced epithelial-to-mesenchymal cell transition via inactivation of PI3K/Akt/?]-catenin signaling in renal tubular epithelial cells.
Kuo YL et al. Sci Rep 2019; 9 (1):10269 Hypoxia-induced epithelial-mesenchymal transition and fibrosis for the development of breast capsular contracture.
Application : WB
Reactivity : Mouse
Hung CY et al. Mol Neurobiol 2019; Sp1 in Astrocyte Is Important for Neurite Outgrowth and Synaptogenesis.
Application : WB
Reactivity : Mouse
Lee DK et al. Biochem Biophys Res Commun 2019; 514 (3):645-652 Cdk5 regulates N-cadherin-dependent neuronal migration during cortical development.
Reactivity : Mouse
Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
Application : WB
Reactivity : Human
Mansel C et al. Neurotoxicol Teratol 2019; 72:58-70 Lead exposure reduces survival, neuronal determination, and differentiation of P19 stem cells.
Application : ICC/IF
Reactivity : Mouse
Chang CY et al. Cell Death Discov 2018; 4:100 PEGylated liposome-encapsulated rhenium-188 radiopharmaceutical inhibits proliferation and epithelial-mesenchymal transition of human head and neck cancer cells in vivo with repeated therapy.
Application : WB
Reactivity : Mouse
DaSilva-Arnold S et al. Mol Hum Reprod 2018; (Epub) heZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation.
Application : WB
Reactivity : Human
Chen CM et al. J Renin Angiotensin Aldosterone Syst 2018; 19 (3):1.47E+15 Hyperglycemia activates the renin-angiotensin system and induces epithelial-mesenchymal transition in streptozotocin-induced diabetic kidneys.
Application : IHC-P
Li JM et al. Ther Adv Med Oncol 2018; 10:1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
Application : WB
Reactivity : Human
Hseu YC et al. J Cell Physiol 2018; 234 (4):4125-4139 Antrodia camphorata inhibits epithelial-to-mesenchymal transition by targeting multiple pathways in triple-negative breast cancers.
Application : WB
Reactivity : Human
Terasaki M et al. Anticancer Res 2018; 38 (4):2169-2179 Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.
Application : WB
Reactivity : Human
Chen CY et al. Int J Mol Sci 2018; 19 (2) Corylin Suppresses Hepatocellular Carcinoma Progression via the Inhibition of Epithelial-Mesenchymal Transition, Mediated by Long Noncoding RNA GAS5.
Application : WB
Reactivity : Human
Shentu TP et al. Sci Rep 2017; 7 (1):18052 Thy-1 dependent uptake of mesenchymal stem cell-derived extracellular vesicles blocks myofibroblastic differentiation.
Application : WB
Reactivity : Human
Chen PC et al. J Cell Physiol 2017; (Epub) Activation of Fibroblasts by Nicotine Promotes the Epithelial-Mesenchymal Transition and Motility of Breast Cancer Cells.
Application : WB
Reactivity : Human
Taglieri L et al. Invest New Drugs 2017; (Epub) The kinesin Eg5 inhibitor K858 induces apoptosis and reverses the malignant invasive phenotype in human glioblastoma cells.
Application : WB
Reactivity : Human
Chou HC et al. Toxicological Sciences 2017; (Epub) L-carnitine via PPARγ- and Sirt1-dependent mechanisms attenuates epithelial–mesenchymal transition and renal fibrosis caused by perfluorooctanesulfonate.
Application : WB
Chan SM et al. Sci Rep 2017; 7 (1):8646 Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells.
Application : WB
Reactivity : Human
Fujiki K et al. J Biol Chem 2017; (Epub) Involvement of Notch1 signaling in malignant progression of A549 cells subjected to prolonged cadmium exposure.
Application : WB
Reactivity : Human
Tong D et al. Cancer Lett 2016; 389:23-32 * inhibits castration-induced emt in prostate cancer by repressing cox2/pge2/stat3 axis.
Application : WB, IHC-P
Reactivity : Human
Jian MY et al. Am J Physiol Lung Cell Mol Physiol 2016; 310 (1):L71-85 N-cadherin coordinates AMP kinase-mediated lung vascular repair.
Application : WB
Reactivity : Rat
Li CH et al. Arch Toxicol 2016; (Epub) Cytoplasmic aryl hydrocarbon receptor regulates glycogen synthase kinase 3 beta, accelerates vimentin degradation, and suppresses epithelial-mesenchymal transition in non-small cell lung cancer cells.
Application : WB
Reactivity : Human
Jheng HF et al. Mol Nutr Food Res 2016; (Epub) Dietary low-fat soy milk powder retards diabetic nephropathy progression via inhibition of renal fibrosis and renal inflammation.
Application : IHC-P
Reactivity : Mouse
Chen LH et al. BMC Cancer 2016; 16:431 Involvement of DNMT 3B promotes epithelial-mesenchymal transition and gene expression profile of invasive head and neck squamous cell carcinomas cell lines.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169