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N-RAS antibody

Overexpression
Anti-N-RAS antibody used in Western Blot (WB). GTX108598
Anti-N-RAS antibody used in Western Blot (WB). GTX108598
Anti-N-RAS antibody used in Western Blot (WB). GTX108598

Cat. No. GTX108598

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all N-RAS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Product Note

Cross-reactivity may occur with other RAS proteins

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Guinea pig, Pig, Xenopus laevis, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human N-Ras Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619748

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NRAS proto-oncogene, GTPase , ALPS4 , CMNS , N-ras , NCMS , NRAS1 , NS6

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side , Golgi apparatus membrane; Lipid-anchor

Background

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-N-RAS antibody used in Western Blot (WB). GTX108598

GTX108598 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with N-RAS antibody (GTX108598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-N-RAS antibody used in Western Blot (WB). GTX108598

GTX108598 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with N-RAS antibody (GTX108598) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-N-RAS antibody used in Western Blot (WB). GTX108598

GTX108598 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with N-RAS antibody (GTX108598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Wu HT et al. Am J Chin Med 2020; 48 (5) : 1221-1241 MLL3 Induced by Luteolin Causes Apoptosis in Tamoxifen-Resistant Breast Cancer Cells through H3K4 Monomethylation and Suppression of the PI3K/AKT/mTOR Pathway.
Application : WB
Reactivity : Human
Tsuboi M et al. J Biol Chem 2017; (Epub) The Transcription Factor HOXB7 Regulates ERK Kinase Activity and Thereby Stimulates the Motility and Invasiveness of Pancreatic Cancer Cells.
Application : WB
Reactivity : Human
Chen CC et al. Cell Signal 2015; 27 (11) : 2182-90 Differentiation and apoptosis induction by * and γ-tocotrienol in HL-60 cells via Ras/ERK/NF-κB and Ras/Akt/NF-κB signaling dependent down-regulation of glyoxalase 1 and HMG-CoA reductase.
Application : WB
Reactivity : Human
Chen CC et al. J Agric Food Chem 2015; Blockade of the Ras/Raf/ERK and Ras/PI3K/Akt Pathways by Monacolin K Reduces the Expression of GLO1 and Induces Apoptosis in U937 Cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169