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N-RAS antibody

Anti-N-RAS antibody used in Western Blot (WB). GTX31543

Cat No. GTX31543

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

A431 Cell Lysate(GTX27909)

Specificity/Sensitivity

N-RAS antibody is predicted to not cross-react with H-RAS

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

N-RAS antibody was raised against a 15 amino acid peptide near the carboxy terminus of human N-RAS. The immunogen corresponding to a region within amino acids 139 and 189 of human N-RAS

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NRAS proto-oncogene, GTPase , ALPS4 , CMNS , N-ras , NCMS , NRAS1 , NS6

Cellular Localization

Cell membrane,Golgi apparatus membrane

Background

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-N-RAS antibody used in Western Blot (WB). GTX31543

GTX31543 WB Image

WB analysis of A431 cell lysate using GTX31543 N-Ras antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339