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N-RAS antibody

Anti-N-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX88676
Anti-N-RAS antibody used in Western Blot (WB). GTX88676

Cat No. GTX88676

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.01-0.03μg/ml
IHC-P 2-3μg/ml

Note :

IHC-P
Human Small Intestine shows staining of cytoplasm and membrane of enterocytes.

Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88676-PEP).

Predict Reactivity

Mouse, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NRAS proto-oncogene, GTPase , ALPS4 , CMNS , N-ras , NCMS , NRAS1 , NS6

Cellular Localization

Cell membrane , Golgi apparatus membrane

Background

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-N-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX88676

GTX88676 IHC-P Image

IHC-P analysis of human small intestine using GTX88676 N-Ras antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-N-RAS antibody used in Western Blot (WB). GTX88676

GTX88676 WB Image

WB analysis of MCF7 cell lysate (35μg protein in RIPA buffer) using GTX88676 N-RAS antibody.
Dilution : 0.5μg/ml

Dilution : 0.5μg/ml

REFERENCE

There are currently no references for N-RAS antibody (GTX88676). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for N-RAS antibody (GTX88676). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf