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N-WASP antibody [GT1328]

Recombinant antibody
Anti-N-WASP antibody [GT1328] used in Western Blot (WB). GTX03240
Anti-N-WASP antibody [GT1328] used in Western Blot (WB). GTX03240
Anti-N-WASP antibody [GT1328] used in Western Blot (WB). GTX03240
Anti-N-WASP antibody [GT1328] used in IHC (Paraffin sections) (IHC-P). GTX03240
Anti-N-WASP antibody [GT1328] used in Western Blot (WB). GTX03240
Anti-N-WASP antibody [GT1328] used in IHC (Paraffin sections) (IHC-P). GTX03240

Cat. No. GTX03240

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1328

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all N-WASP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Observed MW

65 kDa. The observed M.W. is based on the following publication. PMID: 28955984

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human WASL.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WASP like actin nucleation promoting factor , N-WASP , NWASP , WASPB

Cellular Localization

Cytoplasm, cytoskeleton , Nucleus , Cytoplasm

Background

This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-N-WASP antibody [GT1328] used in Western Blot (WB). GTX03240

GTX03240 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with N-WASP antibody [GT1328] (GTX03240) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 28955984

Anti-N-WASP antibody [GT1328] used in Western Blot (WB). GTX03240

GTX03240 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with N-WASP antibody [GT1328] (GTX03240) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 28955984

Anti-N-WASP antibody [GT1328] used in Western Blot (WB). GTX03240

GTX03240 WB Image

WB analysis of various samples using GTX03240 N-WASP antibody [GT1328].
Dilution : 1:1000
Loading : 25μg per lane

Anti-N-WASP antibody [GT1328] used in IHC (Paraffin sections) (IHC-P). GTX03240

GTX03240 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX03240 N-WASP antibody [GT1328].
Dilution : 1:100

Anti-N-WASP antibody [GT1328] used in Western Blot (WB). GTX03240

GTX03240 WB Image

WB analysis of HeLa whole cell lysate using GTX03240 N-WASP antibody [GT1328].
Dilution : 1:1000
Loading : 25μg per lane

Anti-N-WASP antibody [GT1328] used in IHC (Paraffin sections) (IHC-P). GTX03240

GTX03240 IHC-P Image

IHC-P analysis of human placenta tissue section using GTX03240 N-WASP antibody [GT1328].
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 439